Biosynthetic Studies on Okadaic Acid

• Project/Area Number: 24651239
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Living organism molecular science
• Research Institution: The University of Tokyo
• Principal Investigator: ABE Ikuro 東京大学, 薬学研究科(研究院), 教授 (40305496)
• Co-Investigator(Kenkyū-buntansha): AWAKAWA Akayoshi 東京大学, 大学院薬学系研究科, 助教 (80609834)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: オカダ酸 / 生合成 / 遺伝子

Research Abstract

In this study, we tried to identify a gene cluster involved in the biosynthesis of okadaic acid in the dinoflagellate Prorocentrum hoffmanianum. As a result, we succeeded in the PCR amplifications of two PKS genes from the DNA isolated from P. hoffmanianum, and one PKS gene from the chloroplast DNA separated from P. hoffmanianum by using the degenerate primers based on the conserved sequences of bacterial PKSs. We also successfully detected the peptide sequences of the candidate PKSs by LC-orbitrap MS analyses. This is the first detection of a putative PKS proteins from P. hoffmanianum by the proteomic approach. These results will pave the way to future studies to understand the biosynthesis of the complex secondary metabolites in dinoflagellates.

Research Products

• [Journal Article] An environmental bacterial taxon with a large and distinct metabolic repertoire (2014)
  • Author(s): Wilson, M. C., Mori, T., Ruckert, C., Uria, A. R., Helf, M. J., Takada, K., Gernert, C., Steffens, U. A. E., Heycke, N., Schmitt, S., Rinke, C., Helfrich, E. J. N., Brachmann, A. O., Gurgui, C., Wakimoto. T., Kracht, M., Crusemann, M., Hentschel, U., Abe, I., Matsunaga, S., Kalinowski, J., Takeyama, H., Piel, J.
  • Journal Title: Nature Volume: 506 Issue: 7486 Pages: 58-62
  • DOI: 10.1038/nature12959
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 生合成工学による創薬 (2014)
  • Author(s): 阿部郁朗
  • Journal Title: ファルマシア Volume: Vol. 50 Pages: 509-511
  • NAID: 130005160519
  • Peer Reviewed
• [Journal Article] Multiplexing of combinatorial chemistry in antimycin biosynthesis expands the molecular diversity and utility (2013)
  • Author(s): Yan Y., Chen, J., Zhang, L., Zheng, Q., Han, Y., Zhang, H., Awakawa, T., Abe, I., Liu, W.
  • Journal Title: Angew. Chem. Int. Ed. Volume: 52 Issue: 47 Pages: 12308-12312
  • DOI: 10.1002/anie.201305569
  • Peer Reviewed
• [Presentation] LC-MSによる渦鞭毛藻由来ポリケタイド合成酵素の検出 (2013)
  • Author(s): 淡川孝義、Ioana NTAI、佐竹真幸、Neil KELLEHER、橘和夫、阿部郁朗
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2013-03-29
• [Presentation] Expanding the catalytic repertoires of biosynthetic enzyme (2013)
  • Author(s): Abe, I.
  • Organizer: Enzyme Engineering XXII : Emerging topics in Enzyme Engineering
  • Place of Presentation: 富山県富山市
  • Invited
• [Presentation] A bacterial symbiont of Japanese marine sponge Discodermia calyx, produces biologically active metabolites (2013)
  • Author(s): Wakimoto, T., Abe, I.
  • Organizer: XIV International Symposium on Marine Natural Products and 8^<th> European Conference on Marine Natural Products
  • Place of Presentation: La Toja, Galicia, Spain
• [Presentation] Engineered biosynthesis of medicinal natural products (2013)
  • Author(s): Abe, I.
  • Organizer: International Conference on Medicinal Chemistry and Timmerman Award 2013
  • Place of Presentation: Jakarta, Indonesia
  • Invited
• [Presentation] Expanding the catalytic repertoires of biosynthetic enzymes (2013)
  • Author(s): Abe, I.
  • Organizer: Enzyme Engineering XXII: Emerging topics in Enzyme Engineering
  • Place of Presentation: 富山県富山市
  • Invited
• [Presentation] A bacterial symbiont of Japanese marine sponge Discodermia calyx, produces biologically active metabolites (2013)
  • Author(s): Wamkimoto, T., Abe, I.
  • Organizer: XIV International Symposium on Marine Natural Products and 8th European Conference on Marine Natural Products
  • Place of Presentation: La Toja, Galicia, Spain
• [Presentation] LC-MSによる渦鞭毛藻由来ポリケタイド合成酵素の検出
  • Author(s): 淡川孝義、Ioana NTAI、佐竹真幸、Neil KELLEHER、橘和夫、阿部郁朗
  • Organizer: 日本薬学会第１３３年会
  • Place of Presentation: 横浜
• [Book] Natural Products: Discourse, Diversity and Design (2014)
  • Author(s): Abe, I.(分担執筆)
  • Publisher: Wiley
• [Book] Natural Products: Discourse, Diversity and Design（分担執筆） (2014)
  • Author(s): Abe, I.
  • Total Pages: 300
  • Publisher: Wiley
• [Remarks] 東京大学大学院薬学系研究科天然物化学教室
  • URL: http://www.f.u-tokyo.ac.jp/~tennen/head.htm
• [Remarks] 東京大学大学院薬学系研究科天然物化学教室
  • URL: http://www.f.u-tokyo.ac.jp/~tennen/head.htm
• [Remarks] 東京大学大学院薬学系研究科天然物化学教室
  • URL: http://www.f.u-tokyo.ac.jp/~tennen/head.htm