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DISTANCE MEASUREMENTS OF FIBROUS PRION PROTEINS BY PULSE ESR SPECTROSCOPY

Research Project

Project/Area Number 24654112
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Condensed matter physics II
Research InstitutionInstitute for Molecular Science

Principal Investigator

NAKAMURA Toshikazu  分子科学研究所, 物質分子科学研究領域, 准教授 (50245370)

Co-Investigator(Kenkyū-buntansha) YAMAMORI Tohru  北海道大学, 大学院獣医学研究科, 准教授 (00512675)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsプリオン凝集体 / クロイツフェルト・ヤコブ病(CJD) / 狂牛病(ウシ海綿状脳症、BSE) / 電子スピン共鳴法(ESR) / 電子-電子二重共鳴法(DEER) / 電子間相互作用 / 距離計測 / 電子スピン共鳴 / スピンラベル
Research Abstract

This research was performed to clarify the mechanism of formation of the prion filaments. The structure of prion monomer consists of a tertiary C-terminal domain (three alpha-helices, H1, H2 and H3) and an N-terminal random domain. In the experiments, recombinant prion protein with a cysteine mutation was created by E. Coli expression system and the cysteine residue was labeled by nitroxide spin probe. After aggregated prion filaments induced by detergent, the spin-spin interaction was measured by X-band continuous-wave (CW) electron spin resonance (ESR) or Q-band pulse ESR spectrometer. The inter-spin distance was determined by Monte Carlo fitting for electron-electron interaction signals. The inter-spin distance of prion filaments spin-labeled at H1 or H2 is shorter than that of H3. However, the inter-spin distance of N-terminal domain was not estimated because of polydispersity in distance. These data suggested that H1 and H2 were involved in formation of prion filaments.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (26 results)

All 2014 2013 2012 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (18 results) (of which Invited: 1 results) Remarks (1 results)

  • [Journal Article] 3-Methyl pyruvate enhances radiosensitivity through increasing mitochondria-derived reactive oxygen species in tumor cell lines2014

    • Author(s)
      Nishida N, Yasui H, Nagane M, Yamamori T, InanamiO.
    • Journal Title

      J Radiat Res

      Volume: (in press) Issue: 3 Pages: 455-463

    • DOI

      10.1093/jrr/rrt142

    • NAID

      120005457025

    • Related Report
      2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Radiosensitization of tumor cells through endoplasmic reticulum stress induced by PEGylated nanogel containing gold nanoparticles.2014

    • Author(s)
      Yasui H, Takeuchi R, Nagane M, Meike S, Nakamura Y, Yamamori T, Ikenaka Y, Kon Y, Murotani H, Oishi M, Nagasaki Y, Inanami O
    • Journal Title

      Cancer Letters

      Volume: 347 Issue: 1 Pages: 151-158

    • DOI

      10.1016/j.canlet.2014.02.005

    • NAID

      120005425361

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 3-Methyl pyruvate enhances radiosensitivity through increasing mitochondria-derived reactive oxygen species in tumor cell lines.2014

    • Author(s)
      Nishida N, Yasui H, Nagane M, Yamamori T, Inanami O.
    • Journal Title

      J Radiat Res.

      Volume: in press

    • NAID

      120005457025

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Redox factor-1 activates endothelial SIRTUIN1 through reduction of conserved cysteine sulfhydryls in its deacetylase domain.2013

    • Author(s)
      Jung SB, Kim CS, Kim YR, Naqvi A, Yamamori T, Kumar S, Kumar A, Irani K.
    • Journal Title

      PLoS One.

      Volume: 8 Issue: 6 Pages: e65415-e65415

    • DOI

      10.1371/journal.pone.0065415

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] ER stress suppresses DNA double-strand break repair and sensitizes tumor cells to ionizing radiation by stimulating proteasomal degradation of Rad51.2013

    • Author(s)
      Yamamori T, Meike S, Nagane M, Yasui H, Inanami O.
    • Journal Title

      FEBS Lett.

      Volume: 587 Issue: 20 Pages: 3348-3353

    • DOI

      10.1016/j.febslet.2013.08.030

    • NAID

      120005326756

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reoxygenation-induced nitric oxide mitigates tumor hypoxia and radioresistance in a murine SCCVII tumor model2013

    • Author(s)
      Nagane M, Yasui H, Yamamori T, Zhao S, Kuge Y, Tamaki N, Kameya H, Nakamura H, Fujii H, Hirata H, Inanami O
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 437 Issue: 3 Pages: 420-425

    • DOI

      10.1016/j.bbrc.2013.06.093

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The prospective application of a hypoxic radiosensitizer, doranidazole to rat intracranial glioblastoma with blood brain barrier disruption2013

    • Author(s)
      Hironobu Yasui
    • Journal Title

      BMC Cancer

      Volume: 13 Issue: 1 Pages: 106-106

    • DOI

      10.1186/1471-2407-13-106

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Presentation] Longitudinal analysis of tumor oxygenation after the metabolic-targeted treatment with dichloroacetate using pulsed esr imaging2014

    • Author(s)
      Yasui H., Saito K., Nishida N., Matsumoto S., Yamamori T., Krishna M.C., Inanami O.
    • Organizer
      The FIRST Joint International Symposium (Sapporo meeting)/ The 1st GI-CoRE Medical Science and Engineering Symposium
    • Place of Presentation
      Sapporo Park Hotel (Sapporo, Hokkaido)
    • Related Report
      2013 Final Research Report
  • [Presentation] Optimization of the metabolic-targeted radiosensitizing treatment by longitudinal analysis of tumor oxygenation2014

    • Author(s)
      Yasui H., Saito K., Nishida N., Matsumoto S., Yamamori T., Krishna M.C., Inanami O.
    • Organizer
      The 17th Biennial Meeting of Society for Free Radical Research International (SFRRI 2014)
    • Place of Presentation
      Kyoto International Conference Center (Kyoto, Kyoto)
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] LONGITUDINAL ANALYSIS OF TUMOR OXYGENATION AFTER THE METABOLIC-TARGETED TREATMENT WITH DICHLOROACETATE USING PULSED ESR IMAGING2014

    • Author(s)
      Yasui H., Saito K., Nishida N., Matsumoto S., Yamamori T., Krishna M.C., Inanami O
    • Organizer
      The FIRST Joint International Symposium (Sapporo meeting)/ The 1st GI-CoRE Medical Science and Engineering Symposium
    • Place of Presentation
      Sapporo Park Hotel (Sapporo, Hokkaido)
    • Related Report
      2013 Annual Research Report
  • [Presentation] The metabolic-targeted treatment with dichloroacetate transiently decreases tumor oxygenation in a murine squamous cell carcinoma model2013

    • Author(s)
      Yasui H., Saito K., Nishida N., Matsumoto S., Yamamori T., Krishna M.C., Inanami O.
    • Organizer
      The 20th Annual Meeting of the Society for Free Radical Biology and Medicine
    • Place of Presentation
      Sheraton Seattle (Seattle, USA)
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] パルスEPRイメージングを用いたがん代謝標的薬剤ジクロロ酢酸処理後の腫瘍内酸素環境の経時的解析2013

    • Author(s)
      安井博宣、齋藤圭太、西田直哉、松元慎吾、山盛徹、Murali Krishna、稲波修
    • Organizer
      第52回電子スピンサイエンス学会
    • Place of Presentation
      大宮ソニックシティ(さいたま市,埼玉)
    • Related Report
      2013 Final Research Report
  • [Presentation] チャイニーズハムスターV79細胞における硫化水素による放射線防護効果の検討2013

    • Author(s)
      稲波修、山本栄輔、永根大幹、安井博宣、山盛徹
    • Organizer
      日本放射線影響学会第56回大会
    • Place of Presentation
      クラウンパレス青森(青森市,青森)
    • Related Report
      2013 Final Research Report
  • [Presentation] 電子スピン共鳴法を用いた酸素イメージングによる腫瘍内間欠的低酸素の描出とその意義2013

    • Author(s)
      安井博宣、松元慎吾、齋藤圭太、山盛徹、Murali C. Krishna、稲波修
    • Organizer
      日本放射線影響学会第56回大会
    • Place of Presentation
      クラウンパレス青森(青森市,青森)
    • Related Report
      2013 Final Research Report
  • [Presentation] プリオンタンパク質凝集体の電子スピン共鳴-スピンラベル法による解析2013

    • Author(s)
      稲波修、山盛徹、安井博宣、堀内基広、平岡和佳子、古川貢、中村敏和
    • Organizer
      第156回日本獣医学会学術総
    • Place of Presentation
      岐阜大学(岐阜市,岐阜)
    • Related Report
      2013 Final Research Report
  • [Presentation] 放射線による内皮型一酸化窒素合成酵素活性化におけるATMおよびHSP90の関与2013

    • Author(s)
      永根大幹、安井博宣、山盛徹、丹羽光一、稲波修
    • Organizer
      第156回日本獣医学会学術総会
    • Place of Presentation
      岐阜大学(岐阜市,岐阜)
    • Related Report
      2013 Final Research Report
  • [Presentation] 赤血球膜の不安定性を示すウシαスペクトリンの局所的構造変化に関する研究2013

    • Author(s)
      藤本桃子、山盛徹、安井博宣、永根大幹、大津航、木崎皓太、稲葉睦、稲波修
    • Organizer
      第156回日本獣医学会学術総会
    • Place of Presentation
      岐阜大学(岐阜市,岐阜)
    • Related Report
      2013 Final Research Report
  • [Presentation] プリオンタンパク質凝集体のQバンド電子スピン二重共鳴法(Q-band DEER)による構造解析2013

    • Author(s)
      稲波修、山盛徹、安井博宣、堀内基広、平岡和佳子、古川貢、中村敏和
    • Organizer
      第52回電子スピンサイエンス学会年会
    • Place of Presentation
      大宮ソニックシティ (さいたま市, 埼玉県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] プリオンタンパク質凝集体の電子スピン共鳴-スピンラベル法による解析2013

    • Author(s)
      稲波修、山盛徹、安井博宣、堀内基広、平岡和佳子、古川貢、中村敏和
    • Organizer
      第156回日本獣医学会学術総会
    • Place of Presentation
      岐阜大学(岐阜市, 岐阜県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] パルスEPRイメージングを用いたがん代謝標的薬剤ジクロロ酢酸処理後の腫瘍内酸素環境の経時的解析2013

    • Author(s)
      安井博宣、齋藤圭太、西田直哉、松元慎吾、山盛徹、Murali Krishna、稲波修
    • Organizer
      第52回電子スピンサイエンス学会年会
    • Place of Presentation
      大宮ソニックシティ (さいたま市, 埼玉県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] チャイニーズハムスターV79細胞における硫化水素による放射線防護効果の検討2013

    • Author(s)
      稲波修、山本栄輔、永根大幹、安井博宣、山盛徹
    • Organizer
      日本放射線影響学会第56回大会
    • Place of Presentation
      クラウンパレス青森 (青森市, 青森県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 電子スピン共鳴法を用いた酸素イメージングによる腫瘍内間欠的低酸素の描出とその意義2013

    • Author(s)
      安井博宣、松元慎吾、齋藤圭太、山盛徹、Murali C. Krishna、稲波修
    • Organizer
      日本放射線影響学会第56回大会
    • Place of Presentation
      クラウンパレス青森 (青森市, 青森県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 放射線による内皮型一酸化窒素合成酵素活性化におけるATMおよびHSP90の関与2013

    • Author(s)
      永根大幹、安井博宣、山盛徹、丹羽光一、稲波修
    • Organizer
      第156回日本獣医学会学術総会
    • Place of Presentation
      岐阜大学 (岐阜市, 岐阜県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 赤血球膜の不安定性を示すウシαスペクトリンの局所的構造変化に関する研究 第156回日本獣医学会学術総会2013

    • Author(s)
      藤本桃子、山盛徹、安井博宣、永根大幹、大津航、木崎皓太、稲葉睦、稲波修
    • Organizer
      第156回日本獣医学会学術総会
    • Place of Presentation
      岐阜大学 (岐阜市, 岐阜県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Multi-frequency and Pulsed ESR Study on Electric- and Bio-functional Materials2012

    • Author(s)
      Toshikazu Nakamura
    • Organizer
      The 8th Asia-Pacific EPR/ESR Symposium (APES 2012)
    • Place of Presentation
      Tsinghua Science Park in Beijing, China
    • Related Report
      2012 Research-status Report
    • Invited
  • [Remarks]

    • URL

      http://www.ims.ac.jp/know/material/nakamura/nakamura.html

    • Related Report
      2013 Final Research Report

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Published: 2013-05-31   Modified: 2020-05-15  

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