Colloidal crystallization of protein molecules
| Project/Area Number |
24656016
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied materials science/Crystal engineering
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| Research Institution | The University of Tokushima |
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Principal Investigator |
SUZUKI Yoshihisa 徳島大学, ソシオテクノサイエンス研究部, 准教授 (60325248)
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| Co-Investigator(Renkei-kenkyūsha) |
SAKURABA Haruhiko 香川大学, 農学部, 教授 (90205823)
TSUGE Hideaki 京都産業大学, 総合生命科学部, 教授 (40299342)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | コロイド結晶化 / タンパク質結晶 / 遠心濃縮 / コロイド結晶 / タンパク質 / 脱塩 / 液液相分離 |
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| Research Abstract |
We tried to crystalliza protein molecules not via conventional salting-out method but via colloidal crystallization method. Protein molecules were concentrated by centrifugal dialyzattion against ultra-pure water or dilute aqueous HCl solutions.
Unfortunately, we could not obtain typical colloidal crystals of protein. Instead, normal protein crystals without using conventional salting-out method.
In addition, we also confirm novel crystallization processes via liquid-liquid phase separations.
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Report
(3 results)
Research Products
(44 results)
