| Project/Area Number |
24657074
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | Osaka University |
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Principal Investigator |
KOJIMA Chojiro 大阪大学, たんぱく質研究所, 准教授 (50333563)
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| Co-Investigator(Renkei-kenkyūsha) |
FUJIWARA Toshimichi 大阪大学, 蛋白質研究所, 教授 (20242381)
MATSUKI Yoh 大阪大学, 蛋白質研究所, 助教 (70551498)
OHKI Izuru 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (80418574)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | NMR / 構造生物学 / フロリゲン |
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| Research Abstract |
NMR structure can be determined for proteins with molecular weight less than 50 kDa, however, no structure for beyond 100 kDa. In this research, we have developed a new NMR method to analyze very large proteins. This method consists of sensitivity enhancement of 13C direct detection, and 13C assignment and analysis procedures employed for solid state NMR. These methods were applied to florigen receptor with molecular mass beyond 50 kDa.
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