| Project/Area Number |
24658287
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Kyoto University |
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Principal Investigator |
MASUDA Taro 京都大学, (連合)農学研究科(研究院), 助教 (40395653)
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| Co-Investigator(Kenkyū-buntansha) |
MIKAMI Bunzo 京都大学, 大学院農学研究科, 教授 (40135611)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 蛋白質 / 立体構造 / 金属 / リガンド / メタロプロテイン / フェノールオキシダーゼ / キチナーゼ / フェリチン |
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| Outline of Final Research Achievements |
To establish the relationship between a protein and its metal ligand, we divided the research to the following two parts. 1. X-ray crystallographic studies on novel metal binding proteins, and 2. Designing a new metal binding protein based on the structure and function of known metalloproteins. As the results of the first approach, we determined the crystal structures of a copper binding prophenoloxidase from crustacean and a calcium accumulating class III chitinase from pomegranate. Further, we constructed a novel nickel binding protein based on the iron storage protein ferritin.
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