• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Engineering of a hydrocarbon producing yeast for biofuel production

Research Project

Project/Area Number 24658289
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Applied molecular and cellular biology
Research InstitutionNara Women's University

Principal Investigator

KAGIWADA Satoshi  奈良女子大学, 自然科学系, 准教授 (40281662)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsバイオ燃料 / 緑藻 / 酵母 / 炭化水素 / バイオテクノロジー / 再生可能エネルギー / 微生物
Research Abstract

A unicellular green algae, Botryococcus braunii, secretes a hydrocarbon called botryococcene, which is very suitable for biofuel production, into its environment. However, Botryococcus do not grow fast and is not subjected to biotechnological engineering, making it difficult to improve this organism for more efficient biofuel production.
In this study, genes required for botryococcene synthesis are incorporated into the budding yeast Saccharomyces cerevisiae, which is very suitable for biotechnological engineering, to make a yeast strain which can prouduce large amounts of botryococcene and secrete them.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (13 results)

All 2014 2013 2012 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (10 results) Remarks (1 results)

  • [Journal Article] Osh proteins regulate COPII-mediated vesicular transport of ceramide from the endoplasmic reticulum in budding yeast.2014

    • Author(s)
      Kajiwara, K., Ikeda, A., Aguilera-Romero, A., Castillon, G. A., Kagiwada, S., Hanada, K., Riezman, H., Muñiz, M., Funato, K.
    • Journal Title

      Journal of Cell Science

      Volume: 127 Pages: 376-387

    • DOI

      10.1242/jcs.132001

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transformation of lipid bodies related to hydrocarbon accumulation in a green alga, Botryococcus braunii (Race B).2013

    • Author(s)
      Suzuki, R., Ito, N., Uno, Y., Nishii, I., Kagiwada, S., Okada, S. and Noguchi, T
    • Journal Title

      PLoS One

      Volume: 8 Issue: 12 Pages: e81626-e81626

    • DOI

      10.1371/journal.pone.0081626

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Presentation] 緑藻Botryococcus brauniiのトリテルペン合成酵素群の出芽酵母での発現と細胞内局在2013

    • Author(s)
      三井菜々香、野口哲子、鍵和田聡
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      札幌
    • Year and Date
      2013-09-14
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Biosynthesis and accumulation of hydrocarbons and polysaccharides in a colonial green alga, Botryococcus braunii race B2013

    • Author(s)
      Suzuki, R., Uno, Y., Nishii, I., Kagiwada, S. and Noguchi, T.
    • Organizer
      10th International Phycological Congress Annual Meeting
    • Place of Presentation
      Orlando, USA
    • Year and Date
      2013-08-08
    • Related Report
      2013 Final Research Report
  • [Presentation] Biosynthesis and accumulation of lipids and polysaccharides in a colonial green alga, Botryococcus braunii race B2013

    • Author(s)
      Uno, Y., Suzuki, R., Ito, N., Kagiwada, S., Nishii, I. and Noguchi, T.
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Year and Date
      2013-03-23
    • Related Report
      2013 Final Research Report
  • [Presentation] Multi-layered ER induced by ALS8-type Scs2p can be dissolved by modulating phospholipid metabolism in the yeast Saccharomyces cerevisiae2012

    • Author(s)
      Miyake, N., Nakamichi, S. and Kagiwada, S.
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Abundance of the Opi1p transcriptional factor involved in the transcriptional regulation of phospholipid biosynthetic genes is regulated by its binding to membranes in Saccharomyces cerevisiae2012

    • Author(s)
      Oshima, A., Yokoi, R., Masuda, M. and Kagiwada, S.
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-13
    • Related Report
      2013 Final Research Report
  • [Presentation] 緑藻Botryococcus brauniiB品種における脂質の分泌に伴うオイルボディの動態2012

    • Author(s)
      鈴木玲子、宇野由紀、鍵和田聡、西井一郎、野口哲子
    • Organizer
      日本植物学会第76回大会
    • Place of Presentation
      姫路
    • Year and Date
      2012-09-15
    • Related Report
      2013 Final Research Report
  • [Presentation] Hydrocarbon biosynthesis and secretion in a green alga, Botryococcus braunii2012

    • Author(s)
      Hirose, M., Nishii, I., Kagiwada, S. and Noguchi, T.
    • Organizer
      The 2nd International Conference on Algal Biomass, Biofuels and Bioproducts
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2012-06-12
    • Related Report
      2013 Final Research Report
  • [Presentation] Hydrocarbon biosynthesis and secretion in a green alga, Botryococcus braunii

    • Author(s)
      Hirose M, Kagiwada S, Nishii I, Noguchi T
    • Organizer
      International Conference on Algal Biomass, Biofuels and Bioproducts
    • Place of Presentation
      San Diego, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] 緑藻Botryococcus braunii B品種における脂質の分泌に伴うオイルボディの動態

    • Author(s)
      鈴木玲子,宇野由紀,鍵和田聡,西井一郎,野口哲子
    • Organizer
      日本植物学会第76回大会
    • Place of Presentation
      姫路市
    • Related Report
      2012 Research-status Report
  • [Presentation] Biosynthesis and accumulation of lipids and polysaccharides in a colonial green alga, Botryococcus braunii race B

    • Author(s)
      Uno Y, Suzuki R, Ito N, Kagiwada S, Nishii I, Noguchi T
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山市
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://www.nara-wu.ac.jp/rigaku/2014/index.html

    • Related Report
      2013 Final Research Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi