| Project/Area Number |
24659094
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
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| Research Institution | Obihiro University of Agriculture and Veterinary Medicine |
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Principal Investigator |
Ishikawa Toru 帯広畜産大学, 畜産学部, 教授 (70249960)
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| Research Collaborator |
KOTERA Akira 帯広畜産大学, 畜産学部, 学生
KAMADA Takuro 帯広畜産大学, 畜産学部, 学生
NII Takeshi 帯広畜産大学, 畜産学部, 学生
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 起電性ナトリウム重炭酸イオン共輸送体 / pH / マグネシウムイオン / カリウムチャネル / NBCe1-B / Mg2+ / K+チャネル / Kチャンネル / 細胞内Ｎａ / 重炭酸イオン / トランスポーター |
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| Outline of Final Research Achievements |
The main aim of this project was to gain some clues as to the cellular and molecular mechanisms of functional diversity of the electrogenic sodium bicarbonate cotransporter NBCe1-B observed in various native tissues and cell types. Using a heterologous mammalian expression system, H+ was suggested to be a cytosolic factor that might modify the intracellular Mg2+-sensitivity of NBCe1-B. Studies on ion channels in bovine parotid acinar cells endogenously expressing NBCe1-B may raise the possibilities that pH-sensitive K+ currents and TRPM6/7-like cation channel activity might be involved, at least in part, in regulation of NBCe1-B in these cells. The data obtained in this project will be useful for future studies to better understand regulatory mechanisms of the electrogenic sodium bicarbonate cotransporter activity in intact cells.
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