Mechanism of carcinogenesis by bacterial pathogenic factor

• Project/Area Number: 24659120
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Hokkaido University
• Principal Investigator: HIGASHI Hideaki 北海道大学, 人獣共通感染症リサーチセンター, 教授 (20311227)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 細菌 / タンパク質リン酸化 / 細菌感染 / 発がん / タンパク質 / 感染症

Outline of Final Research Achievements

As infection with intestinal residential bacteria depends on the host immune system and induces tumor formation, bacterial infection was predicted to be a potential risk factor of carcinogenesis, as is the case for oncoviral infection. The aim of this study was to reveal the effect of bacterial infection on cell proliferation through the functional analysis of Tarp molecules in Chlamydia trachomatis. We created a humanized C. trachomatis tarp gene and successfully constructed a system for ectopic Tarp expression in animal cells, which has been considered difficult thus far. We also studied an approach to create genetically modified animals using the humanized genes.

Research Products

• [Journal Article] A novel multiplex PCR discriminates Bacillus anthracis and its genetically related strains from other Bacillus cereus group species (2015)
  • Author(s): Ogawa H., Fujikura D., Ohnuma M., Ohnishi N., Hang'ombe B.M., Mimuro M., Ezaki T., Mweene A.S., Higashi H
  • Journal Title: PLoS One Volume: 10 Issue: 3 Pages: e0122004-e0122004
  • DOI: 10.1371/journal.pone.0122004
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Bacillus cereus from the environment is genetically related to the highly pathogenic B. cereus in Zambia (2015)
  • Author(s): Ogawa H., Ohnuma M., Squarre D., Mweene AS. , Ezaki T., Fujikura D., Ohnishi N., Thomas Y., Hang’ombe BM., Higashi H.
  • Journal Title: J VET MED SCI Volume: 印刷中
  • NAID: 130005094678
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Pathological and molecular diagnosis of the 2013 African swine fever outbreak in Lusaka, Zambia (2015)
  • Author(s): Yabe J, Hamambulu P, Simulundu E, Ogawa H, Kajihara M, Mori-Kajihara A, Changula-Chitanga K, Mwase M, Mweemba-Muwowo M, Chambaro HM, Mataa L, Hang'ombe B, Namangala B, Fandamu P, Sawa H, Takada A, Higashi H, Mweene AS.
  • Journal Title: Trop Anim Health Prod Volume: 47 Issue: 2 Pages: 459-463
  • DOI: 10.1007/s11250-014-0732-0
  • Peer Reviewed / Open Access
• [Journal Article] Research Activities of Hokudai Center for Zoonosis Control in Zambia. (2014)
  • Author(s): Higashi H., Kida, H.
  • Journal Title: Journal of Disaster Research Volume: 9 Pages: 818-822
  • NAID: 130007673774
  • Peer Reviewed
• [Journal Article] Effects of single injection of local anesthetic agents on intervertebral disc degeneration: ex vivo and long-term in vivo experimental study (2014)
  • Author(s): Iwasaki K, Sudo H, Yamada K, Higashi H, Ohnishi T, Tsujimoto T, Iwasaki N.
  • Journal Title: PLoS One Volume: 9 Issue: 10 Pages: e109851-e109851
  • DOI: 10.1371/journal.pone.0109851
  • NAID: 120005527839
  • Peer Reviewed / Open Access
• [Journal Article] Genome Sequence of Bacillus anthracis outbreak strain in Zambia (2014)
  • Author(s): Ohnishi N., Maruyama F., Ogawa H., Kachi H., Yamada S., Fujikura D., Nakagawa I., Hang'ombe B.M., Thomas Y., Mweene A.S., Higashi H.
  • Journal Title: 2011. Genome Announc Volume: 2 Issue: 2
  • DOI: 10.1128/genomea.00116-14
  • Peer Reviewed / Open Access
• [Journal Article] Caspase 3 Silencing Inhibits Biomechanical Overload-Induced Intervertebral Disk Degeneration (2014)
  • Author(s): Yamada K., Sudo H., Iwasaki K., Sasaki N., Higashi H., Kameda Y., Ito M., Takahata M., Abumi K., Minami A., Iwasaki N.
  • Journal Title: Am. J. Pathol. Volume: 184 Issue: 3 Pages: 753-764
  • DOI: 10.1016/j.ajpath.2013.11.010
  • Peer Reviewed
• [Journal Article] Global identification of genes related to nutrient deficiency in intervertebral disc cells in an experimental nutrient deprivation mode. (2013)
  • Author(s): Sudo H., Yamada K., Iwasaki K., Higashi H., Ito M., Minami A., Iwasaki N.
  • Journal Title: PLoS One Volume: 8
  • Peer Reviewed
• [Journal Article] Tertiary structure-function analysis reveals the pathogenic signaling potentiation mechanism of Helicobacter pylori oncogenic effector CagA (2012)
  • Author(s): Hayashi T., Senda M., Morohashi H., Higashi H., Horio M., Kashiba Y., Nagase L., Sasaya D., Shimizu T., Venugopalan N., Kumeta H., Noda N.N., Inagaki F., Senda T., Hatakeyama M.
  • Journal Title: Cell Host Microbe Volume: 12 Issue: 1 Pages: 20-33
  • DOI: 10.1016/j.chom.2012.05.010
  • Peer Reviewed
• [Journal Article] Human - Animal Anthrax outbreak in the Luangwa valley of Zambia in 2011 (2012)
  • Author(s): Hang'ombe B.M., Mwansa J.C.L., Muwowo S., Mulenga P., Kapina M., Musenga E., Squarre D., Mataa L., Thomas S.Y., Ogawa H., Sawa H., Higashi H.
  • Journal Title: Trop. Doct. Volume: 42 Issue: 3 Pages: 136-139
  • DOI: 10.1258/td.2012.110454
  • Peer Reviewed
• [Presentation] Optimal Career Decisions by Young Researchers (2014)
  • Author(s): 東 秀明
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: タワーホール船堀（東京）
  • Invited
• [Presentation] Control and prevention of anthrax in human and animals (2013)
  • Author(s): Hideaki Higashi
  • Organizer: International Conference in Medicine and Public Health 2013
  • Place of Presentation: Mahidol University（Thailand）
  • Invited
• [Presentation] De novo designed molecule to develop structure-based vaccine against Anthrax (2013)
  • Author(s): 大西 なおみ、武藤 芽未、藤倉 大輔、五十嵐 学、東 秀明
  • Organizer: 第60回毒素シンポジウム
  • Place of Presentation: 楓香荘（兵庫）
• [Presentation] 炭疽のコントロールを目指して ーアフリカにおける炭疽サーベイランスとワクチン開発ー (2013)
  • Author(s): Hideaki Higashi
  • Organizer: 第13回日本バイオセーフティ学会総会
  • Place of Presentation: 北海道大学（札幌）
  • Invited
• [Presentation] De novo designed molecule to develop structure-based vaccine against (2013)
  • Author(s): 東 秀明
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 幕張メッセ（千葉県）
• [Presentation] Development of de novo designated molecular for structure-based Anthrax vaccine (2013)
  • Author(s): Hideaki Higashi
  • Organizer: Asian-African Research Forum on Emerging and Reemerging Infections 2013
  • Place of Presentation: 東京医科歯科大学（東京都）