Establishment of the scoring system for predicting the prognosis of CKD patients.

• Project/Area Number: 24659279
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Laboratory medicine
• Research Institution: The University of Tokushima
• Principal Investigator: ABE Naoko 徳島大学, 大学病院, 特任助教 (70623271)
• Co-Investigator(Kenkyū-buntansha): DOI Toshio 徳島大学, ヘルスバイオサイエンス研究部, 教授 (60183498)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: CKD / 慢性腎臓病 / 予後 / バイオマーカー / 予後予測マーカー / podocyte / エクソゾーム

Research Abstract

The worldwide rise in the number of patients with chronic kidney disease (CKD) and consequent end-stage renal failure is a global public health problem. Current screening strategies, based on creatinine or albuminuria, fail to identify a considerable number of subjects with CKD. We first isolate exosomes from various CKD patients. We then examined the expression of podocyte-derived exosomal transcriptional factors (PDETFs) in urine from various CKD patients with gross proteinuria. While extent of albuminuria was not different, progressing renal diseases such as diabetic nephropathy exhibited high levels of urinary PDETFs. Glomerular expression of PDETFs was decreased in accordance with the amount of PDETFs in urine of the patients. The higher amount of urinary PDETFs secreted, the earlier the renal function declined. Evaluation and analysis of PDETFs would provide some clues to understanding the molecular linkage between CKD and CVD.

Research Products

• [Journal Article] Dual involvement of growth arrest-specific gene 6 in the early phase of human IgA nephropathy. (2013)
  • Author(s): Nagai K1, Miyoshi M, Kake T, Fukushima N, Matsuura M, Shibata E, Yamada S, Yoshikawa K, Kanayama HO, Fukawa T, Yamaguchi K, Izaki H, Mima A, Abe N, Araoka T, Murakami T, Kishi F, Kishi S, Tominaga T, Moriya T, Abe H, Doi T.
  • Journal Title: PLoS One Volume: 8(6) Issue: 6 Pages: e66759-e66759
  • DOI: 10.1371/journal.pone.0066759
  • Peer Reviewed
• [Journal Article] Scleraxis modulates bone morphogenetic protein 4 (BMP4)-Smad1-smooth muscleαactin (SMA) signal transduction in diabetic nephropathy (2012)
  • Author(s): Abe H, Tominaga T, Matsubara T, Abe N, Kishi S, Nagai K, Murakami T, Araoka T, Doi T.
  • Journal Title: J Biol Chem Volume: 287(24) Pages: 20430-20442
  • Peer Reviewed
• [Journal Article] Scleraxis modulates bone morphogenetic protein 4 (BMP4)-Smad1-smooth muscle α actin(SMA) signal transduction in diabetic nephropathy (2012)
  • Author(s): Abe H, Tominaga T, Matsubara T, Abe N, Kishi S, Nagai K, Murakami T, Araoka T, Doi T.
  • Journal Title: J Biol Chem Volume: 287 Issue: 24 Pages: 20430-20442
  • DOI: 10.1074/jbc.m111.275610
  • Peer Reviewed
• [Presentation] Multiple regulatory pathways of BMP-Smad axis through a sclerosis-specific basic HLH transcription factor in diabetic nephropathy (2013)
  • Author(s): Yui Fujita, Akiko Sakurai, Tatsuya Tominaga, Sanae Hayashi, Naoko Abe, Toshio Doi, Hideharu Abe
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市)
• [Presentation] 腎機能低下予測バイオマーカーとしてのポドサイト由来エクソソーム内転写因子 (2013)
  • Author(s): 安部尚子、安部秀斉、土井俊夫
  • Organizer: 第53回日本臨床化学会年次学術集会
  • Place of Presentation: あわぎんホール(徳島県徳島市)
• [Presentation] 腎機能低下予測バイオマーカーとしてのポドサイト由来エクソソーム内転写因子 (2013)
  • Author(s): 安部尚子、土井俊夫、安部秀斉
  • Organizer: 第53回 日本臨床化学会年次学術集会
  • Place of Presentation: あわぎんホール（徳島県徳島市）
• [Presentation] Predictive biomarkers for kidney function declines : podocyte-derived exosomal transcriptional factors (2012)
  • Author(s): 安部尚子、林早苗、土井俊夫、安部秀斉
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡(福岡県福岡市)
• [Presentation] Predictive biomarkers for kidney function declines: podocyte-derived exosomal transcriptional factors (2012)
  • Author(s): 安部尚子、他
  • Organizer: 第35回 日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場（福岡県）
• [Remarks]
  • URL: http://www.tokudai-kidney.jp/achievement/index.htm