| Project/Area Number |
24659474
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
膠原病・アレルギー・感染症内科学
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| Research Institution | The University of Tokushima |
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Principal Investigator |
FUKUI Hiroyuki 徳島大学, ヘルスバイオサイエンス研究部, 教授 (90112052)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKEDA Noriaki 徳島大学, 大学院ヘルスバイオサイエンス研究部, 教授 (30206982)
MIZUGUCHI Hiroyuki 徳島大学, 大学院ヘルスバイオサイエンス研究部, 准教授 (40247838)
KITAMURA Yoshiaki 徳島大学, 大学院ヘルスバイオサイエンス研究部, 講師 (60380028)
FUJII Tatsuya 徳島大学, 大学院ヘルスバイオサイエンス研究部, 特任助教 (00724048)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | アレルギー性鼻炎 / 花粉症 / 好酸球 / IL-33 / プロテインキナーゼC / アレルギー疾患 / PKCδ / 抗アレルギー天然物 / 疾患感受性遺伝子 |
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| Research Abstract |
We observed the close relationship between levels of IL-33 mRNA in nasal mucosa and blood eosinophil in pollinosis patients. We succeeded in finding Swiss3T3 cells in which IL-33 mRNA level was up-regulated by the stimulation of PMA. Then we observed that PMA-induced up-regulation of IL-33 mRNA was strongly suppressed by the extract of Kujin, an anti-allergic Kampo medicine. (-)Maackiain, an inhibitor of PKC-delta from Kujin revealed to be a suppressor. Up-regulation of IL-33 mRNA was also suppressed by either rottlerin, a PKC-delta inhibitor, quercetin, another natural PKC-delta inhibitor or 17-AAG, a suppressor of heat shock protein-90. IL-33 mRNA up-regulation was suppressed by U0126, a MEK inhibitor, but not by DPQ, a PARP-1 inhibitor.
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