Molecular design of microbial surfactants
| Project/Area Number |
24681013
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Environmental technology/Environmental materials
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| Research Institution | Kitami Institute of Technology |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥26,520,000 (Direct Cost: ¥20,400,000、Indirect Cost: ¥6,120,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
Fiscal Year 2012: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Keywords | 糖脂質 / 酵母 / 真菌 / ゲノム解析 / 培養 / バイオプロダクション / 微生物 / 遺伝子組換え / 形質転換 / バイオサーファクタント / 代謝工学 / ドラフトゲノム解析 / Pseudozyma / Rhodococcus / 放線菌 |
|---|
| Outline of Final Research Achievements |
To identify glycolipid-synthesizing genes, draft genome sequencing was carried out for a glycolipid producing fungi, Pseudozyma hubeiensis SY62. Seventy four scaffolds were obtained from approximately 60,000,000 read of raw data. The total length of scaffolds reached to approximately 18 Mb. MEL synthetic genes were identified by comparing of genome sequences between SY62 and Ustilago maydis 521. Then, transformation method was developed using a vector including green fluorescent protein constructed from Ustilago-Escherichia coli shuttle vector, pUXV1.
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Report
(4 results)
Research Products
(18 results)
