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A single mRNA counting by a microchamber array device containing a single human nucleus

Research Project

Project/Area Number 24681027
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypePartial Multi-year Fund
Research Field Microdevices/Nanodevices
Research InstitutionNagoya University

Principal Investigator

KAJI Noritada  名古屋大学, 工学(系)研究科(研究院), 准教授 (90402479)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥27,170,000 (Direct Cost: ¥20,900,000、Indirect Cost: ¥6,270,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2012: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Keywordsナノバイオ / mRNA / 細胞
Outline of Final Research Achievements

In this research, a microchamber array device was designed to capture a single cell, lyse a cell membrane, and isolate a single cell nucleus, and then mRNA in a single cell nucleus were directly quantitated by using highly sensitive In-Stem Molecular Beacons (ISMB). As a result, the expression of three frequently used house-keeping genes were successfully quantitated to an accuracy of 100 molecules within a single cell nucleus.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (39 results)

All 2015 2014 2013 2012

All Journal Article (11 results) (of which Peer Reviewed: 11 results,  Open Access: 3 results) Presentation (23 results) (of which Invited: 11 results) Book (4 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Self-assembled Nanowire Arrays as Three-dimensional Nanopores for Filtration of DNA Molecules2015

    • Author(s)
      S. Rahong, T. Yasui, T. Yanagida, K. Nagashima, M. Kanai, G. Meng, Y. He, F. Zhuge, N. Kaji, T. Kawai, Y. Baba
    • Journal Title

      Analytical Sciences

      Volume: 31 Issue: 3 Pages: 153-157

    • DOI

      10.2116/analsci.31.153

    • NAID

      130004827469

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Microfluidic transfer of liquid interface for parallel stretching and stamping of terminal-unmodified single DNA molecules in zigzag-shaped microgrooves2014

    • Author(s)
      H. Yasaki, D. Onoshima, T. Yasui, H. Yukawa, N. Kaji, and Y. Baba
    • Journal Title

      Lab Chip

      Volume: 15 Issue: 1 Pages: 135-140

    • DOI

      10.1039/c4lc00990h

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Novel positively charged nanoparticle labeling for in vivo imaging of adipose tissue-derived stem cells.2014

    • Author(s)
      Yukawa H, Nakagawa S, Yoshizumi Y, Watanabe M, Saito H, Miyamoto Y, Noguchi H, Oishi K, Ono K, Sawada M, Kato I, Onoshima D, Obayashi M, Hayashi Y, Kaji N, Ishikawa T, Hayashi S, Baba Y.
    • Journal Title

      PLoS One.

      Volume: 9 Issue: 11 Pages: e110142-e110142

    • DOI

      10.1371/journal.pone.0110142

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ultrafast and wide range analysis of DNA molecules using rigid network structure of solid nanowires2014

    • Author(s)
      S. Rahong, T. Yasui, T. Yanagida, K. Nagashima, M. Kanai, A. Klamchuen, G. Meng, Y. He, F. Zhuge, N. Kaji, T. Kawai, Y. Baba
    • Journal Title

      Scientific Reports

      Volume: 4 Issue: 1 Pages: 5252-5252

    • DOI

      10.1038/srep05252

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Induced Pluripotent Stem Cell Labeling Using Quantum Dots2013

    • Author(s)
      H. Yukawa, K. Suzuki, Y. Kano, T. Yamada, N. Kaji, T. Ishikawa, Y. Baba
    • Journal Title

      Cell Medicine

      Volume: 6 Issue: 1-2 Pages: 83-90

    • DOI

      10.3727/215517913x674270

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA Manipulation and Separation in Sublithographic-Scale Nanowire Array2013

    • Author(s)
      Takao YASUI, Sakon RAHONG, Koki MOTOYAMA, Takeshi YANAGIDA, Qiong WU, Noritada KAJI, Masaki KANAI, Kentaro DOI, Kazuki NAGASHIMA, Masanobu TOKESHI, Masateru TANIGUCHI, Satoyuki KAWANO, Tomoji KAWAI, and Yoshinobu BABA
    • Journal Title

      ACS Nano

      Volume: 7 Issue: 4 Pages: 3029-3035

    • DOI

      10.1021/nn4002424

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A microfluidic device for quantitative analysis of chemoattraction in plants2013

    • Author(s)
      1. Mitsuhiro Horade, Masahiro M Kanaoka, Motoki Kuzuya, Tetsuya Higashiyama, Noritada Kaji
    • Journal Title

      RSC Advances

      Volume: 3 Issue: 44 Pages: 22301-22307

    • DOI

      10.1039/c3ra42804d

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enzyme-catalysed reaction for long-term fluorescent observation of single DNA molecules2013

    • Author(s)
      T. Yasui
    • Journal Title

      RSC Advances

      Volume: 3 Issue: 10 Pages: 3237-3240

    • DOI

      10.1039/c3ra22999h

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nanobiodevices for biomolecule analysis and imaging2013

    • Author(s)
      T. Yasui
    • Journal Title

      Annual review of analytical chemistry

      Volume: (印刷中) Issue: 1 Pages: 83-96

    • DOI

      10.1146/annurev-anchem-062012-092619

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nanopillar array chip integrated with on-line stacking for fast DNA separation with high sensitivity and high resolution2012

    • Author(s)
      T. Yasui
    • Journal Title

      Microfluidics and Nanofluidics

      Volume: 14 Issue: 6 Pages: 1-7

    • DOI

      10.1007/s10404-012-1103-7

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fabrication of functionalized double-lamellar multifunctional envelope-type nanodevices using a microfluidic chip with a chaotic mixer array2012

    • Author(s)
      K. Kitazoe
    • Journal Title

      PLoS ONE

      Volume: 7 Issue: 6 Pages: e39057-e39057

    • DOI

      10.1371/journal.pone.0039057

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] DNA separation by nanopillars and nanowires2015

    • Author(s)
      Noritada Kaji, Takao Yasui, Yoshinobu Baba
    • Organizer
      International workshop on extended-nano fluidics
    • Place of Presentation
      Tokyo
    • Year and Date
      2015-03-27
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] マイクロウェルを用いた単一細胞解析法の開発2015

    • Author(s)
      小山諒、加地範匡、安井隆雄、馬場嘉信
    • Organizer
      日本化学会 第95春季年会
    • Place of Presentation
      千葉
    • Year and Date
      2015-03-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] ナノ流路を用いた単一分子解析技術2015

    • Author(s)
      加地範匡、安井隆雄、馬場嘉信
    • Organizer
      先端ナノデバイス・材料テクノロジー第151委員会 ナノバイオフュージョン分科会 第7回 合同研究会
    • Place of Presentation
      東京
    • Year and Date
      2015-03-06
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Nanopillar chips for a Millisecond DNA and RNA separations2014

    • Author(s)
      Noritada Kaji, Qiong Wu, Takao Yasui, Yoshinobu Baba
    • Organizer
      14th Asia-Pacific International Symposium on Microscale Separations and Analysis (APCE2014)
    • Place of Presentation
      Kyoto
    • Year and Date
      2014-12-08
    • Related Report
      2014 Annual Research Report
  • [Presentation] Rapid fabrication and purification of multifunctional envelope-type gene delivery nanodevices by microfluidic devices2014

    • Author(s)
      Noritada Kaji, Takao Yasui, Yoshinobu Baba
    • Organizer
      8th International Symposium on Nanomedicine (ISNM2014)
    • Place of Presentation
      Matsuyama
    • Year and Date
      2014-12-05
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ナノ構造体を用いたナノバイオデバイスの研究開発2014

    • Author(s)
      加地範匡
    • Organizer
      第18回VBLシンポジウム
    • Place of Presentation
      名古屋
    • Year and Date
      2014-11-17
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] A Millisecond Micro-RNA Extraction Technique for Nanopore-based Nucleic Acids Sequencing2014

    • Author(s)
      Qiong Wu, Takao Yasui, Sakon Rahong, Takeshi Yanagida, Masaki Kanai, Noritada Kaji, Manabu Tokeshi, Kazuki Nagashima, Tomoji Kawai, Yoshinobu Baba
    • Organizer
      The 18th International Conference on Miniaturized System for Chemistry and Life Sciences (uTAS2014)
    • Place of Presentation
      San Antonio, Texas
    • Year and Date
      2014-10-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] Nanobiocevices for single DNA and cell analysis2014

    • Author(s)
      Noritada Kaji, Yoshinobu Baba
    • Organizer
      The 18th International Conference on Miniaturized System for Chemistry and Life Sciences (uTAS2014)
    • Place of Presentation
      San Antonio, Texas
    • Year and Date
      2014-10-29
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Nanowires, Nanopillars, and Nanoslits as a Pretreatment Tool for Nanopore-based DNA Sequencing2014

    • Author(s)
      Noritada Kaji, Yoshinobu Baba
    • Organizer
      4th Annual Next Generation Sequencing and 2nd Annual Single Cell Genomics Asia Congress
    • Place of Presentation
      Singapore
    • Year and Date
      2014-10-07
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ナノ流路におけるDNA速度制御法の開発2014

    • Author(s)
      孫暁寅、加地範匡、安井隆雄、馬場嘉信
    • Organizer
      日本分析化学会第63年会
    • Place of Presentation
      広島
    • Year and Date
      2014-09-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] ナノワイヤデバイスによるバイオ分析 ~ナノポアDNAシーケンシングのための核酸前処理行程の開発~2014

    • Author(s)
      加地範匡
    • Organizer
      ナノバイオ国際共同研究教育拠点 第3回若手国内シンポジウム NanoBio第7回若手ネットワーキングシンポジウム
    • Place of Presentation
      福岡
    • Year and Date
      2014-06-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] ナノピラー、ナノワイヤ、ナノスリットによる統合型核酸解析デバイスの開発2014

    • Author(s)
      加地範匡、安井隆雄、馬場嘉信
    • Organizer
      第21回クロマトグラフィーシンポジウム
    • Place of Presentation
      名古屋
    • Year and Date
      2014-06-06
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Nano- and Quantum-biodevices for Cancer Diagnosis, Cancer Therapy, and iPS Cell Based Regenerative Medicine2014

    • Author(s)
      Noritada Kaji, Yoshinobu Baba
    • Organizer
      Advances in Microfluidics and Nanofluidics (AMN2014)
    • Place of Presentation
      Taipei
    • Year and Date
      2014-05-21
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] A Single DNA Manipulation and Analysis in Nanofluidic Devices2014

    • Author(s)
      Noritada Kaji, Takao Yasui, Yoshinobu Baba
    • Organizer
      Advances in Microfluidics and Nanofluidics (AMN2014)
    • Place of Presentation
      Taipei
    • Year and Date
      2014-05-21
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] マイクロチャンバーを用いた単一核解析法の解析2013

    • Author(s)
      加地範匡、小山諒、安井隆雄、馬場嘉信
    • Organizer
      日本分析化学会第62年会
    • Place of Presentation
      大阪
    • Related Report
      2013 Annual Research Report
  • [Presentation] ナノバイオデバイスによる単一生体分子解析2013

    • Author(s)
      加地範匡、安井隆雄、馬場嘉信
    • Organizer
      第7回バイオ関連化学シンポジウム
    • Place of Presentation
      名古屋
    • Related Report
      2013 Annual Research Report
  • [Presentation] ナノワイヤデバイスによるバイオ分析2013

    • Author(s)
      加地範匡
    • Organizer
      ナノバイオ国際共同研究教育拠点 第2回若手国内シンポジウム NanoBio第6回若手ネットワーキングシンポジウム
    • Place of Presentation
      富山
    • Related Report
      2013 Annual Research Report
  • [Presentation] A new design of microfluidic components for bioanalysis2013

    • Author(s)
      Toyohiro Naito, Noritada Kaji, Yoshinobu Baba
    • Organizer
      5th International symposium on microchemistry and microsystems (ISMM2013)
    • Place of Presentation
      Xiamen, CHINA
    • Related Report
      2013 Annual Research Report
  • [Presentation] High speed and highly sensitive biomolecule measurements by nanopillar- and nanopore-based nanobiodevices2013

    • Author(s)
      Noritada Kaji
    • Organizer
      17th KAST International Symposium on "The Multi-Omics and Nano Biotechnology for Human Disease Research"
    • Place of Presentation
      Seoul, KOREA
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Micro and Nanochamber Array Chip for a Single Cell, Nucleus, and Protein Analysis2013

    • Author(s)
      Noritada Kaji, Takao Yasui, Yoshinobu Baba
    • Organizer
      International Conference on Small Science (ICSS 2013)
    • Place of Presentation
      Las Vegas, NV
    • Related Report
      2013 Annual Research Report
  • [Presentation] Nanobiodevices-based single biomolecule and single cell analysis for cancer diagnosis/therapy and stem cell therapy2013

    • Author(s)
      Noritada Kaji, Yoshinobu Baba
    • Organizer
      LAB-ON-A-CHIP ASIA 2013
    • Place of Presentation
      Singapore
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] "大きな技術"で小さな生体分子を視る・操る2012

    • Author(s)
      加地範匡
    • Organizer
      さかえサイエンストーク
    • Place of Presentation
      名古屋(招待講演)
    • Year and Date
      2012-10-01
    • Related Report
      2012 Annual Research Report
  • [Presentation] Micro- and Nanofabricated Structures on a Chip for Biomolecule Analysis in Artificial Intracellular Environments2012

    • Author(s)
      Noritada Kaji
    • Organizer
      ISMM2012
    • Place of Presentation
      Hsinchu, Taiwan(招待講演)
    • Year and Date
      2012-06-11
    • Related Report
      2012 Annual Research Report
  • [Book] Nanotechnology and Plant Sciences (Carbon Nanotubes and Modern Nanoagriculture)2015

    • Author(s)
      Maged F Serag, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
    • Total Pages
      19
    • Publisher
      Springer
    • Related Report
      2014 Annual Research Report
  • [Book] 現代化学(超解像蛍光顕微鏡の開発)2014

    • Author(s)
      加地範匡、渡慶次学、馬場嘉信
    • Total Pages
      4
    • Publisher
      東京化学同人
    • Related Report
      2014 Annual Research Report
  • [Book] ぶんせき(ナノ流路を用いた単一分子解析技術)2014

    • Author(s)
      加地範匡、安井隆雄、馬場嘉信
    • Total Pages
      7
    • Publisher
      日本分析化学会
    • Related Report
      2014 Annual Research Report
  • [Book] 化学と教育、精密にサイズ制御したナノ空間内での分析化学2013

    • Author(s)
      加地範匡
    • Total Pages
      2
    • Publisher
      日本化学会
    • Related Report
      2013 Annual Research Report
  • [Patent(Industrial Property Rights)] 電気測定用チップ、及び電気測定装置2014

    • Inventor(s)
      矢崎啓寿、安井隆雄、加地範匡、川合知二、柳田剛、馬場嘉信
    • Industrial Property Rights Holder
      矢崎啓寿、安井隆雄、加地範匡、川合知二、柳田剛、馬場嘉信
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-214090
    • Filing Date
      2014-10-20
    • Related Report
      2014 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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