3D tissue construction based on "Cell fibers"
| Project/Area Number |
24686031
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Intelligent mechanics/Mechanical systems
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| Research Institution | Keio University (2013-2014)
The University of Tokyo (2012) |
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Principal Investigator |
ONOE Hiroaki 慶應義塾大学, 理工学部, 講師 (30548681)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥26,650,000 (Direct Cost: ¥20,500,000、Indirect Cost: ¥6,150,000)
Fiscal Year 2014: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2012: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
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| Keywords | マイクロ流体 / 細胞 / 組織工学 / ハイドロゲル / マイクロファイバ / MEMS / ３次元組織 / マイクロファイバー / 3次元組織 / 移植医療 |
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| Outline of Final Research Achievements |
The aim of this project is to develop a fiber-shaped building unit, named “cell fiber,” for the construction of centimeter-scale 3D tissue constructs. I fabricated cell fibers that have a core of cell-suspended extracellular matrix (ECM) hydrogel and a shell of Ca-alginate hydrogel by using a double co-axial laminar flow microfluidic device. I found out that the choice of core ECM materials is critical to form cell fibers corresponding to various types of cells. Using cell fibers, I demonstrated a fiber-based centimeter-scale 3D tissue constructs woven by our developed micro weaving machine, and bundle-like neural 3D tissue structures. Those fabricated fiber-based 3D constructs maintained their viability and can be applied to in vivo implantation, which suggest that this fiber-based approach would be a powerful method for constructing large-scale spatially-ordered functional tissues.
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Report
(4 results)
Research Products
(32 results)
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[Presentation] Neural matchsticks for 3D neural network assembly2014
Author(s)
Midori. Kato-Negishi, Hiroaki Onoe, Akiko Sato, Shoji Takeuchi
Organizer
Eighteenth International Conference on Miniaturized Systems for Chemistry and Life Science (microTAS)
Place of Presentation
San Antonio (USA)
Year and Date
2014-10-26 – 2014-10-30
Related Report
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[Presentation] Survival of neural stem/progenitor cells is promoted when cells are loaded in artificial microfibers in mouse complete spinal cord injury model2014
Author(s)
Keiko Hori, Soraya Nishimura, Midori Kato-Negishi, Hiroaki Onoe, Yoshiomi Kobayashi, Go Itakura, Hiroki Iwai, Shoji Takeuchi, Hideyuki Okano, Yoshiaki Toyama, Masaya Nakamura
Organizer
The 53rd ISCoS Annual Scientific Meeting
Place of Presentation
Maastricht (The Netherlands)
Year and Date
2014-09-02 – 2014-09-04
Related Report
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[Presentation] Neural stem/progenitor cells-laden microfibers promote survival of transplants in mouse transected spinal cord injury model2014
Author(s)
Keiko Hori, Soraya Nishimura, Midori Kato-Negishi, Hiroaki Onoe, Yoshiomi Kobayashi, Go Itakura, Hiroki Iwai, Shoji Takeuchi, Hideyuki Okano, Yoshiaki Toyama, Masaya Nakamura
Organizer
ISSCR (International Society for Stem Cell Research) 12th Annual Meeting
Place of Presentation
Vancouver (Canada)
Year and Date
2014-06-18 – 2014-06-21
Related Report
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[Presentation] Neural stem/progenitor cells-laden microfibers promote cellular survival in mouse transected spinal cord injury model2013
Author(s)
Keiko Hori, Soraya Nishimura, Midori Negishi, Hiroaki Onoe, Yoshiomi Kobayashi, Go Itakura, Hiroki Iwai, Shoji Takeuchi, Hideyuki Okano, Yoshiaki Toyama, Masaya Nakamura
Organizer
Society for Neuroscience 2013 Annual Meeting
Place of Presentation
San Diego (USA)
Year and Date
2013-11-09 – 2013-11-13
Related Report
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