| Budget Amount *help |
¥27,820,000 (Direct Cost: ¥21,400,000、Indirect Cost: ¥6,420,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2012: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
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| Outline of Final Research Achievements |
We have carried out developments for technological tools to investigate single-molecule gene expression dynamics in single cells of higher organisms in a high-throughput manner. To realize this, we have developed a light-sheet illumination fluorescence microscopy that enables 3D volumetric imaging within the whole volume of thick cells set on a multi-well plate. In addition, we have developed a probing technique to detect mRNA and protein expressions at single molecule levels. Through analysis of gene expression dynamics in Saccharomyces cerevisiae cells for multiple gene targets, we have found a common feature in mechanisms on gene expression noise.
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