Role of enteric bacteria on adaptation to low protein diet

• Project/Area Number: 24687032
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Physical anthropology
• Research Institution: Jikei University School of Medicine
• Principal Investigator: Iwase Tadayuki 東京慈恵会医科大学, 医学部, 講師 (80385294)
• Project Period (FY): 2012-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2015: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2013: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
• Keywords: 細菌学 / 人類学 / 栄養学 / 進化・適応 / 腸内細菌 / 窒素代謝 / 宿主－細菌相互作用 / 栄養・生理学 / 学際

Outline of Final Research Achievements

We designed and developed an incubator for 15N-gas exposure experiments with a domestic manufacturer. Mice with or without nitrogen-fixing bacteria were kept for 3 days under 15N gas in the incubator. The internal organs (intestinal contents, intestinal tract, liver, body hair) of the mice were harvested and analyzed nitrogen Isotope ratio of the samples using an elemental analysis / isotope ratio mass spectrometer to measure. The results indicated that a statistically significant increase in the nitrogen isotope ratio was found in intestinal content, intestinal tract, and liver. In contrast, no significance was observed in the body hair.

Research Products

• [Journal Article] Complete Genome Sequence of Klebsiella oxytoca Strain JKo3. (2016)
  • Author(s): Iwase T, Ogura Y, Hayashi T, Mizunoe Y.
  • Journal Title: Genome Announc Volume: 4 Issue: 6
  • DOI: 10.1128/genomea.01221-16
  • Peer Reviewed / Open Access
• [Journal Article] Complete Genome Sequence of Klebsiella pneumoniae YH43. (2016)
  • Author(s): Iwase T, Ogura Y, Hayashi T, Mizunoe Y.
  • Journal Title: Genome Announc Volume: 4 Issue: 2
  • DOI: 10.1128/genomea.00242-16
  • Peer Reviewed / Open Access
• [Journal Article] Microbe-microbe and host-microbes interactions (2013)
  • Author(s): Iwase T, Mizunoe Y.
  • Journal Title: Seikagaku Volume: 85(9) Pages: 803-806
  • NAID: 10031202195
• [Journal Article] A simple assay for measuring catalase activity: a visual approach. (2013)
  • Author(s): Iwase T, Tajima A, Sugimoto S, Okuda K, Hironaka I, Kamata Y, Takada K, Mizunoe Y.
  • Journal Title: Sci Rep Volume: 3 Issue: 1 Pages: 3081-3081
  • DOI: 10.1038/srep03081
  • Peer Reviewed
• [Journal Article] 宿主―病原細菌―常在細菌間の相互作用 (2013)
  • Author(s): 岩瀬忠行、水之江義充
  • Journal Title: 実験医学 Volume: 30 Pages: 125-133
• [Presentation] 腸管出血性大腸菌 O157 の臨床分離株で見出さ れた一塩基変異による RpoS の機能不全 (2016)
  • Author(s): 岩瀬 忠行，田嶌 亜紀子，水之江 義充
  • Organizer: 第89回日本細菌学会総会
  • Place of Presentation: 大阪府大阪市
  • Year and Date: 2016-03-23
• [Presentation] ペースメーカーより分離されたPropionibacterium acnes の分子系統解析とバイオフィルム形成 (2016)
  • Author(s): 奥田 賢一, 山田 聡美, 杉本 真也, 岩瀬 忠行, 佐藤 真理, 佐藤 主税, 水之江 義充
  • Organizer: 第89回日本細菌学会総会
  • Place of Presentation: 大阪府大阪市
  • Year and Date: 2016-03-23
• [Presentation] バイオフィルム感染症制圧の試み (2016)
  • Author(s): 水之江 義充, 奥田 賢一, 岩瀬 忠行, 千葉 明生, 杉本 真也
  • Organizer: 電子情報通信学会 有機エレクトロニクス研究会
  • Place of Presentation: 熊本県熊本市
  • Year and Date: 2016-01-08
  • Invited
• [Presentation] 細菌のバイオフィルムマトリクスの解析 (2015)
  • Author(s): 水之江 義充, 千葉 明生, 岩瀬 忠行, 杉本 真也
  • Organizer: 電子情報通信学会ソサイエティ大会・界面ナノバイオテクノロジーシンポジウム
  • Place of Presentation: 宮城県仙台市
  • Year and Date: 2015-09-08
  • Invited
• [Presentation] ペースメーカーより分離されたPropionibacterium acnesによるバイオフィルム形成 (2015)
  • Author(s): 奥田 賢一, 杉本 真也, 岩瀬 忠行, 山田 聡美, 水之江 義充
  • Organizer: 日本バイオフィルム学会
  • Place of Presentation: 愛知県蒲郡市
  • Year and Date: 2015-07-10
• [Presentation] 哺乳類腸内における窒素固定 (2015)
  • Author(s): 岩瀬 忠行
  • Organizer: 安定同位体医学応用研究基盤拠点の形成「第5回研究報告会」
  • Place of Presentation: 東京慈恵会医科大学
  • Year and Date: 2015-03-07
  • Invited
• [Book] 病原菌の今日的意味（改訂5版） (2016)
  • Author(s): 水之江 義充，岩瀬 忠行
  • Publisher: 株式会社 医薬ジャーナル社