| Budget Amount *help |
¥24,830,000 (Direct Cost: ¥19,100,000、Indirect Cost: ¥5,730,000)
Fiscal Year 2015: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Fiscal Year 2012: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
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| Outline of Final Research Achievements |
I investigated function of miR-223 and Foxo1 in skin wound healing. Skin wound healing in miR-223 KO mice was delayed concomitant with altered neutrophil activation. I showed that miR-223 KO mice undergo prolonged acute inflammatory responses at wound sites due to miR-223 KO-derived neutrophils exhibiting increased hyperoxidative bursts. Intriguingly, S. aureus-infected miR-223 KO mice showed markedly faster skin wound healing than S. aureus-infected wild-type mice. Our data indicate that the molecular targeting of miR-223 may regulate the healing of bacterium-infected chronic skin wounds.
Foxo1 heterozygous mice led to accelerated skin wound healing with enhanced keratinocyte migration and reduced scarring accompanied by an attenuated inflammatory response. Transient knockdown of Foxo1 at the wound site by local delivery of antisense oligo enhanced skin wound healing. Overall, molecular targeting of FOXO1 may improve the quality of healing and reduce pathological scarring.
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