Role of clutch protein shootin1 in activity-dependent axonal elongation
Project/Area Number |
24700349
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Neuroscience in general
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Research Institution | Tokyo Metropolitan Institute of Medical Science |
Principal Investigator |
SHIMADA Tadayuki 公益財団法人東京都医学総合研究所, 脳発達・神経再生分野, 主席研究員 (80379552)
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Project Period (FY) |
2012-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | てんかん / 軸索分枝 / 神経回路 / FGFシグナル / 軸索分枝形成 / 神経可塑性 / FGF / 神経活動 / 神経回路網 / 軸索伸長、分枝形成 / 神経細胞 |
Outline of Final Research Achievements |
Because we could not find the evidence that shootin1 is involved in the aberrant axonal branch formation of hippocampal granule cells after epileptic seizure, we investigated the function of activity-dependent proteins in seizure-mediated axonal branching. We found that overexpression of neuritin, one of the protein increased after the seizures, promoted axonal branching in granule cells. Chemical kindling induced less severe seizures in neuritin knockout mice than in wild-type mice, and neuritin knockout mice showed smaller number of axonal branching after the induction of epileptic seizures. We found that excess amount of Neuritin recruited FGF receptors to cell surface and the recruitment of FGF receptors activated FGF signaling. Furthermore, the increased activity of FGF signaling promoted axonal branching in granule cells. We concluded that Neuritin-mediated translocation of FGF receptors induce abberant axonal branching after epileptic seizures.
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Report
(5 results)
Research Products
(17 results)
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[Journal Article] Collapsin response mediator protein 4 regulates growth cone dynamics through the actin and microtubule cytoskeleton2014
Author(s)
Mohamad R. Khazaei, Marie-Pier Girouard, Ricardo Alchini, Stephan Ong Tone, Tadayuki Shimada, Susanne Bechstedt, Mitra Cowan, Dominique Guillet, Paul W. Wiseman, Gary Brouhard, Jean Francois Cloutier and Alyson E. Fournier.
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Journal Title
Journal of Biochemistry
Volume: 289
Issue: 43
Pages: 30133-30143
DOI
Related Report
Peer Reviewed
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[Journal Article] Activation of Rheb, but not of mTORC1, impairs spine synapse morphogenesis in tuberous sclerosis complex.2014
Author(s)
Yasuda S, Sugiura H, Katsurabayashi S, Shimada T, Tanaka H, Takasaki K, Iwasaki K, Kobayashi T, Hino O, Yamagata K.
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Journal Title
Sci Rep.
Volume: 4
Issue: 1
Pages: 5155-5155
DOI
Related Report
Peer Reviewed / Open Access
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