| Project/Area Number |
24700698
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Sports science
|
|---|
| Research Institution | The University of Tokushima |
|---|
Principal Investigator |
MIYAKE Masato 徳島大学, 疾患プロテオゲノム研究センター, 助教 (30588976)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
|---|
| Keywords | 骨格筋 / 肥満 / 小胞体 / エネルギー消費 / 褐色脂肪 / ホルモン / 臓器連関 / 小胞体ストレス / アミノ酸代謝 |
|---|
| Research Abstract |
In skeletal muscle, some physiological stimulation, for example exercise activated PERK-eIF2alpha phosphorylation signal. Fv2E-PERK is artificial gene that is activated by ligand. We generated muscle-specific Fv2E-PERK expressing transgenic (TG) mice. Adiposity of TG mice fed high-fat diet was decreased compared from wild type. TG mice also improved insulin sensitivity and oxygen consumption. However, metabolic profile in skeletal muscle seemed to be same between wild type and TG mice. Surprisingly, gene expression related to energy expenditure was higher in brown adipose tissue of TG mice than in that of wild type mice. Some experiments showed that PERK-eIF2alpha phosphorylation single regulated anti obesity hormone FGF21 expression in skeletal muscle. Plasma concentration of FGF21 also increased in TG mice. These results suggest that FGF21 induced by eIF2alpha phosphorylation in skeletal muscle increases energy expenditure by affecting on brown adipose tissue.
|
