A new method to evaluate parytoxin content in sea foods
| Project/Area Number |
24700857
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Eating habits, studies on eating habits
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| Research Institution | Shokei Junior College |
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Principal Investigator |
SAGARA Takefumi 尚絅大学短期大学部, その他部局等, 講師 (60353132)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | パリトキシン / C6グリオーマ細胞 / PC12細胞 / LC-MS / アオブダイ / ハコフグ / 細胞毒性 / 横紋筋融解症 / 食品衛生 / 培養細胞 / 質量分析 |
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| Outline of Final Research Achievements |
In this project, I investigated to develop a new method using cultured cell to evaluate palytoxin content. As the results, a rat C6 glioma cell was suitable to use for a toxic evaluation for the palytoxin, furthermore I conclude that the method of detection by LDH release was sensitive. In addition, the method was applicable to the test solution that I prepared from toxic specimen Scarus ovifrons. On the other hand, peaks were detected in chromatogram of m/z 327.6, 1352.3 and 1360.3 in LC-MS analysis of the test solution. However did not come to conclude that it was the peak to originate from palytoxin or a palytoxin-like substance because the influence was inarticulate peak shape.
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Report
(4 results)
Research Products
(2 results)
