Environmental factor for microcystin degradative activity in freshwater environment

• Project/Area Number: 24710081
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Environmental technology/Environmental materials
• Research Institution: Toyo University
• Principal Investigator: SHIMIZU Kazuya 東洋大学, 生命科学部, 講師 (10581613)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 淡水資源 / 浄水処理法 / 藍藻類由来有毒物質 / 生物膜法 / 水圏現象 / 微生物機能 / 生物膜処理

Outline of Final Research Achievements

In the present study, environmental factor for microcystin degradative activity and enzymatic pathway of microcystin degradation in microcystin degrading bacterium were revealed. In aquatic environment, the relationship between microcystin degradation and microcystinase gene, mlrA is one to one. This relationship is very interesting because most of the cases are not one to one relation. The mlrA gene is unique. The rapid microcystin degradation in aquatic environment and drinking water treatment system is supposed that microcystin sequential degradation is occurred by interaction between microcystin degrading bacteria and microcystin degradation related microorganisms in microbial community.

Research Products

• [Journal Article] Determination of microcystin-LR degrading gene mlrA in biofilms at a biological drinking water treatment facility (2013)
  • Author(s): Shimizu Kazuya, Maseda Hideaki, Okano Kunihiro, Hiratsuka Takumi, Jimbo Yusuke, Xue Qiang, Akasako Haruna, Itayama Tomoaki, Utsumi Motoo, Zhang Zhenya, and Sugiura Norio
  • Journal Title: Maejo International Journal of Science and Technology Volume: 7 (Special Issue)
  • Peer Reviewed
• [Journal Article] Enzymatic pathway for biodegrading microcystin LR in Sphingopyxis sp. C-1 (2012)
  • Author(s): Shimizu Kazuya, Maseda Hideaki, Okano Kunihiro, Kurashima Takumi, Kawauchi Yukio, Xue Qiang, Utsumi Motoo, Zhang Zhenya, and Sugiura Norio
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 114 Pages: 630-634
  • NAID: 110009578157
  • Peer Reviewed
• [Presentation] 生物学的浄水処理法における藍藻由来有毒物質分解および分解酵素遺伝子の動態
  • Author(s): 清水和哉、赤迫春菜、李潔明、内海真生、杉浦則夫
  • Organizer: 第19回日本環境毒性学会研究発表会
  • Place of Presentation: 東洋大学白山キャンパス（東京）
• [Presentation] Seasonal analysis of microcystin degradation by biological treatment facility at a water purification plant in Japan
  • Author(s): Kazuya Shimizu, Motoo Utsumi, Haruna Akasako, Jieming Li, Hideaki Maseda, and Norio Sugiura
  • Organizer: SETAC 34th North America
  • Place of Presentation: Nashville, TN, USA
• [Remarks] 東洋大学生命科学部応用生物科学科分子生態学研究室webサイト
  • URL: http://www2.toyo.ac.jp/~k_shimizu/ri_ben_yu/JPN-TOP.html
• [Remarks] 分子生態学研究室
  • URL: http://www2.toyo.ac.jp/~k_shimizu/synthetic_ecology/welcome.html