Development of novel culture technique for neural stem cells using polyacrylic acid-grafted fabrics
| Project/Area Number |
24760652
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biofunction/Bioprocess
|
|---|
| Research Institution | Osaka Prefecture University |
|---|
Principal Investigator |
MORI Hideki 大阪府立大学, 理学(系)研究科(研究院), 助教 (30450894)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
|---|
| Keywords | neural stem cell / Nylon / fabric / cell culture / spheroid / 細胞培養 / 神経幹細胞 / 高分子繊維 / 放射線 / グラフト重合 / stem cell / ポリアミド |
|---|
| Outline of Final Research Achievements |
Acrylic acid (AAc) was grafted onto Nylon (PA66) fabrics through graft polymerization induced by gamma-ray pre-irradiation. Activation of the carboxyl groups in poly AAc (PAAc) induced by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)/ N-hydroxyscuccinimide (NHS) treatment allowed covalent immobilization of proteins (Matrigel, etc.). The effects of the PAAc-graftred and protein-immobilized fabrics on the adherence and differentiation of mouse neural stem/progenitor cells (NSPCs) were investigated. Matrigel-immobilized PA66 fabric was the best for the growth of NSPCs in the experimental fabrics.
|
Report
(4 results)
Research Products
(9 results)
