| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Research Abstract |
Lignocellulosic biomass passes through the process of three steps: pretreatment, enzymatic hydrolysis and ethanol fermentation. During the process of hydrothermal pretreatment that is one of the pretreatment, fermentation inhibitors are generated from lignocellulosic biomass. In this study, we analyzed quantification of various fermentation inhibitors derived from hydrothermal pretreatment. Furthermore, we investigated an effect of fermentation inhibitors on yeast growth and fermentation. As a result, furfural, 5-HMF and formic acid affected both yeast growth and ethanol fermentation under the same condition. However, in the case of acetic acid, inhibition of fermentation was not caused by high concentration though the rate of yeast growth decreased at the same condition. Moreover, we determined the effects of inhibition substance on yeast growth by fitting to Monod equation. Also, we quantified the effect of these inhibitors on the Monod parameters.
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