Spatial and temporal variations in adaptive genes: from genome analysis to population dynamics

• Project/Area Number: 24770014
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ecology/Environment
• Research Institution: Nihon University (2014-2015); The University of Tokyo (2012-2013)
• Principal Investigator: MORINAGA Shin-Ichi 日本大学, 生物資源科学部, 助教 (80568262)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ゲノム / 適応遺伝子 / シロイヌナズナ近縁種 / 植物標本 / 生物標本 / 時空間変動 / 適応 / 標本 / 個体群動態

Outline of Final Research Achievements

To investigate the spatial and temporal variations in adaptive genes, I conducted population genomic analysis for current and past individuals in Arabidopsis halleri subsp. gemmifera that grows in Mt. Ibuki, Mt. Fujiwara, and other populations in Japan. I established a reference genome by de novo assembly analysis and employed population genomic analysis of spatial and temporal variations in adaptive genes for each 56 current and 200 past individuals. Based on these results, I discussed the differences in the number, kind, and function of adaptive genes among populations, and the evolutionary responses to global and local environmental changes in wild plant populations.

Research Products

• [Journal Article] From the laboratory to the field: assaying histone methylation at FLOWERING LOCUS C in naturally growing Arabidopsis halleri (2016)
  • Author(s): Nishio, H., Buzas, D. M., Nagano, A. J., Suzuki, Y., Sugano, S., Ito, M., Morinaga, S.-I., Kudoh, H.
  • Journal Title: Genes & Genetic Systems Volume: 91
  • NAID: 130005165463
  • Peer Reviewed
• [Journal Article] A genome scan for genes underlying microgeographic-scale local adaptation in a wild Arabidopsis species (2015)
  • Author(s): Kubota, S., Iwasaki, T., Hanada, K., Nagano, A. J., Toyoda, A., Fujiyama, A., Sugano, S., Suzuki, Y., Hikosaka, K., Ito, M., and Morinaga, S.-I.
  • Journal Title: PLOS Genetics Volume: 11 Issue: 7 Pages: e1005361-e1005361
  • DOI: 10.1371/journal.pgen.1005361
  • NAID: 120005947027
  • Peer Reviewed / Open Access
• [Journal Article] Poor correlation between the removal or deposition of pollen grains and frequency of pollinator contact with sex organs (2013)
  • Author(s): Sakamoto, R.L., Morinaga, S.-I.
  • Journal Title: Naturwissenschaften Volume: 100 Issue: 9 Pages: 871-876
  • DOI: 10.1007/s00114-013-1087-5
  • Peer Reviewed
• [Journal Article] Fine-scale flower-visiting behavior revealed by using a high-speed camera. (2012)
  • Author(s): Sakamoto, R.L., Morinaga. S., Ito M, Kawakubo, N.
  • Journal Title: Behavioral Ecology and Sociobiology Volume: 66 Issue: 5 Pages: 1-6
  • DOI: 10.1007/s00265-011-1314-z
  • Peer Reviewed
• [Presentation] ゲノム情報を利用した野生植物の適応力多様性評価 (2016)
  • Author(s): 久保田渉誠・岩崎貴也・三浦憲人・永野惇・花田耕介・松葉史紗子・宮下直・彦坂幸毅・伊藤元己・森長真一
  • Organizer: 第63回日本生態学会大会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2016-03-24
• [Presentation] UV-B-induced damage on DNA and growth in highland and lowland ecotypes of Arabidopsis species (2015)
  • Author(s): Qing-Wei Wang・尾崎洋史・永野聡一郎・森長真一・日出間純・彦坂幸毅
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 朱鷺メッセ新潟（新潟県新潟市）
  • Year and Date: 2015-09-07
• [Presentation] 大根野生種ハマダイコンにおけるトランスクリプトーム解析 (2015)
  • Author(s): 田島直幸・石塚航・花田耕介・清水みなみ・鈴木穣・伊藤元己・彦坂幸毅・森長真一
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 朱鷺メッセ新潟（新潟県新潟市）
  • Year and Date: 2015-09-07
• [Presentation] ゲノムワイドSNPデータに基づくハクサンハタザオの系統地理解析 (2015)
  • Author(s): 久保田渉誠・岩崎貴也・三浦憲人・永野惇・花田耕介・彦坂幸毅・伊藤元己・森長真一
  • Organizer: 第62回日本生態学会大会
  • Place of Presentation: 鹿児島大学（鹿児島県・鹿児島市）
  • Year and Date: 2015-03-21
• [Presentation] 標本ゲノム解析から局所適応の時間的スケールを計る (2014)
  • Author(s): 久保田渉誠・岩崎貴也・永野惇・花田耕介・彦坂幸毅・伊藤元己・森長真一
  • Organizer: 第46回種生物学シンポジウム
  • Place of Presentation: 富士Calm人材開発センター富士研修所（山梨県・富士吉田市）
  • Year and Date: 2014-12-06
• [Presentation] Eco-evolutionary genomics of spatial and temporal variation in Arabidopsis
  • Author(s): Morinaga, S.-I.
  • Organizer: The 84th Annual Meeting of Genetic Society of Japan
  • Place of Presentation: Kyushu University
  • Invited
• [Presentation] ゲノムの時空間変異で迫るシロイヌナズナ近縁種の生態進化
  • Author(s): 森長真一
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学
• [Presentation] シロイヌナズナ近縁種の局所適応と短期的進化：ゲノムを温ねて生態を知る
  • Author(s): 森長真一
  • Organizer: 日本進化学会第14回大会
  • Place of Presentation: 首都大学東京
  • Invited
• [Presentation] ゲノムの時空間変異から迫るシロイヌナズナ近縁種の生態進化
  • Author(s): 森長真一
  • Organizer: 第60回日本生態学会大会
  • Place of Presentation: 静岡市グランシップ
  • Invited
• [Book] Eco-evolutionary genomic observation for local and global environmental changes (2014)
  • Author(s): Morinaga, S.-I., T. Iwasaki, and Y. Suyama
  • Total Pages: 9
  • Publisher: Springer
• [Book] Eco-evolutionary genomic observation for local and global environmental changes. “The biodiversity observation network in the Asia-Pacific Region: Integrative Observations and Assessments of Asian Biodiversity.” Edited by S. Nakano, T. Yahara, T. Nakashizuka”. (2014)
  • Author(s): Morinaga, S.-I., T. Iwasaki, and Y. Suyama
  • Publisher: Springer
• [Book] エコゲノミクス 遺伝子からみた適応 (2012)
  • Author(s): 森長真一・工藤洋 編
  • Total Pages: 302
  • Publisher: 共立出版