Elucidating the double fertilization mechanism controlled by sperm membrane structures
| Project/Area Number |
24770062
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphology/Structure
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| Research Institution | The University of Tokyo (2014)
Waseda University (2012-2013) |
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Principal Investigator |
MORI Toshiyuki 東京大学, 理学(系)研究科(研究院), 特任研究員 (00462739)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 重複受精 / 配偶子 / 有性生殖 / 花粉 / 被子植物 / GCS1 / GEX2 / 膜タンパク質 |
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| Outline of Final Research Achievements |
This study aimed to analyze gamete interaction during double fertilization specific to flowering plants using molecular cell biological strategies. Especially, this study focused on elucidating the molecular functions of transmembrane proteins identified in male gametes. When a proteomics analysis of Lilium generative cell membrane was performed based on the RNA sequencing data, a novel transmembrane protein named LGM1 was newly detected. LGM1 proved to be a small molecule, which possesses four transmembrane domains and conserved in algae, moss and lycophytes. As a result of molecular cytological analyses of LGM1, it was found that LGM1 is expressed specifically in male gametes.
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] Sex-specific post-translational regulation of the gamete fusogen GCSI in the isogamous volvocine alga Gonium pectorale.2014
Author(s)
Kawai-Toyooka H, Mori T, Hamaji T, Suzuki M, Olson BJ, Uemura T, Ueda T, Nakano A, Toyoda A, Fujiyama A, Nozaki H
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Journal Title
EUkaryotic Cell
Volume: (in press)
Issue: 5
Pages: 648-656
DOI
Related Report
Peer Reviewed
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