| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Research Abstract |
The self-assembly mechanism for rearrangement of actin cytoskeleton plays important roles in cell polarization. In this study, I investigated the mechanosensitive rearrangement of actin cytoskeleton by an actin polymerization factor, mDia1. Fluorescence single-molecule speckle microscopy show that processive actin assembly by mDia1 is stimulated by microneedle micromanipulation. This mDia1 activation is suppressed by inactivation of an actin depolymerization factor, cofilin. s-FDAP analysis show that its cytoplasmic actin monomer concentration is increased by about 10% after the micromanipulation. These results suggest a self-assembly system including a micromanipulation-induced actin disassembly, an increase in the actin monomer concentration, processive actin assembly by mDia1 and the rearrangement of actin cytoskeleton.
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