Functional analysis of non-coding RNA in regulation of chromosome segregation
| Project/Area Number |
24770168
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Kumamoto University |
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Principal Investigator |
IDEUE Takashi 熊本大学, 自然科学研究科, 助教 (20420250)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | RNA / 染色体分離 / ヘテロクロマチン / non-coding RNA / Aurora B / セントロメア / RBMX / 細胞周期 / non coding RNA |
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| Research Abstract |
Satellite I RNA is noncoding RNA transcribed from human centromeric repetitive sequence. Depletion of this RNA caused the abnormal nuclear morphology due to defects in chromosome segregation. We founded that Aurora kinase B, a key factor of chromosome segregation binds to this RNA. Aurora B was regulated kinase activity and localization through binding to this RNA. To identify interaction factors to satellite I RNA, we performed pulled down of Satellite I RNA. Several interactors were identified from pulled down products by LC/MS analysis. Depletion of RBMX, one of interaction protein, showed phenotype of defect in chromosome segregation.
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Report
(3 results)
Research Products
(35 results)
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[Presentation] Involvement of novel complex consisting of the solacing factor Prp14/DHX38 RNA helices and centromeric non-coding RNAs in the regulation of chromatin segregation2013
Author(s)
M. Mutazono, T. Ideue, M. Morita, K. Nishimura, Y. Cho, C. Tsukahara, M. Chinen, J. Nakayama, K. Ishii, T. Tani
Organizer
18th annual meeting of the RNA society-2013
Place of Presentation
Davos, Switzeland
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