• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Novel shRNA library screening to identify key factors that are important in pluripotent stem cells

Research Project

Project/Area Number 24770206
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Developmental biology
Research InstitutionKyoto University

Principal Investigator

HOTTA Akitsu  京都大学, iPS細胞研究所, 特定拠点助教 (50578002)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsshRNAライブラリー / トランスポゾンベクター / 発現抑制制御 / 発現抑制
Outline of Final Research Achievements

To reveal the molecular mechanisms behind the pluripotency of ES cells and iPS cells, we constructed a piggyBac DNA transposon vector to deliver a genome-wide shRNA library into ES/iPS cells. The generated shRNA library was validated by deep sequencing, and several ten-thousand shRNAs were successfully cloned into our piggyBac vector. Our piggyBac shRNA library is a versatile tool to dissect the molecular mechanisms in pluripotent stem cells and other cell types, without the use of viral vectors.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (2 results)

All 2014 2013

All Journal Article (1 results) Presentation (1 results) (of which Invited: 1 results)

  • [Journal Article] 細胞種変換を支える遺伝子導入技術2014

    • Author(s)
      堀田秋津
    • Journal Title

      細胞

      Volume: 46(5) Pages: 220-224

    • Related Report
      2013 Research-status Report
  • [Presentation] トランスポゾン発現抑制を司る因子群のshRNAライブラリーによる全ゲノム規模探索2013

    • Author(s)
      堀田秋津、仲埜峻雄、白井紗矢、笹川典子、藤本直子、Sylvie Rival-Gervier、山本卓也、山中伸弥
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Invited

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi