Molecular mechanisms linking nutritional and metabolic status to neural tube closure

• Project/Area Number: 24770207
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Kyoto University
• Principal Investigator: KUSAKABE MORIOH 京都大学, 生命科学研究科, 助教 (80378843)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 発生・分化 / シグナル伝達

Research Abstract

Although epidemiological studies have suggested that defects in neural tube closure are associated with metabolic status, the underlying molecular mechanism(s) remains elusive. We found that the metabolic sensor AMPK is essential for anterior neural tube closure in Xenopus. Our biochemical experiments revealed that a cytoskeleton-regulating protein is an AMPK substrate in vivo. These results suggest the possibility that AMPK links the metabolic sensing pathway to the cytoskeleton-regulating machinery in neural tube closure.

Research Products

• [Presentation] Analysis of the function of genes that are involved in Xenopus early development (2014)
  • Author(s): Chie Shintani,Toshiyasu Suzuki,Tetsuro Araki,Isabel de Ridder,Morioh Kusakabe,and Eisuke Nishida
  • Organizer: The 12th International Student Seminar Kyoto University
  • Place of Presentation: Kyoto,Japan (ポスター発表 (発表日 : 2014年2月17日))
• [Presentation] アフリカツメガエルの初期匹において神経発生に関わる新規因子の探索 (2013)
  • Author(s): 新谷千栄、鈴木俊康、荒木徹朗、de Ridder Lsabel、日下部杜央、西田栄介
  • Organizer: 第36回日本分子生物学学会
  • Place of Presentation: 神戸ポートアイランド・神戸・日本 (ポスター発表 (発表日 : 2013年12月4日))
• [Presentation] Identification and characterization of Xenopus kctd15,an FGF-repressed ectodermal gene ]SDB-BSDB&BSCB (2012)
  • Author(s): Chika Takahashi,Toshiyasu Suzuki,Eisuke Nishida and Morioh Kusakabe
  • Organizer: Joint meeting of Developmental Biology
  • Place of Presentation: The University of Warwick,Coventry,UK (ポスター発表 (発表日 : 2012年4月15日))
• [Presentation] Identification and characterization of Xenopus kctd15, an FGF-repressed ectodermal gene (2012)
  • Author(s): Takahashi C, Suzuki T, Nishida E, Kusakabe M.
  • Organizer: JSDB-BSDB&BSCB Joint meeting of Developmental Biology
  • Place of Presentation: University of Warwick, Coventry, UK
• [Remarks]
  • URL: http://www.lif.kyoto-u.ac.jp/labs/signal/