| Project/Area Number |
24770230
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Evolutionary biology
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| Research Institution | Josai University (2013-2014)
Sophia University (2012) |
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Principal Investigator |
SANO Kaori 城西大学, 理学部, 助手 (70612092)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 新規機能獲得遺伝子 / 孵化酵素 / 機能進化 / ZPタンパク質 / 卵膜 / 分子進化 |
|---|
| Outline of Final Research Achievements |
In teleosts, hatching enzyme gene diversification has occurred in the common ancestor of Otocephala and Euteleostei, such that members of these subdivisions possess two types of hatching enzyme genes, belonging to clade I and clade II. Our previous study suggested that the clade I enzyme maintained the ancestral function, while the clade II enzyme gained new function (neofunctionalization). Ｍedaka Oryzias latipes ,belonging to Euteleostei, hatch by cooperative action of clade I and II enzymes, and the substrate specificities of them are completely different. To elucidate the amino acid substitutions important for change of substrate specificity, the recombinant clade I enzyme which was substituted nine of the two hundreds amino acids to clade II enzyme type (clade I mut9) was generated by E. coli expression system. The clade I mut9 changed the substrate specificity to be similar to that of clade II enzyme, and cleaved the peptides designed from clade II enzyme specific cleavage sites.
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