| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The aim of this study is the increasing of transformation frequency in non-model plants. To increase the transformation frequency, we attempt to improve the Agrobacterium tumefaciens. Plant synthesize the ethylene and GABA during co-cultivation, and these compounds prevent Agrobacterium from transforming T-DNA. Therefore in this study, we try to breeding new Agrobacterium tumefaciens with the ability to reduce GABA. GABA were degraded by GABA transaminase, however the Agrobacterium tumefaciens does not have the enzyme. We isolated the GABA transaminase from Eschelichia coli and introduce the gene using with plasmid. We produced two types of Agrobacterium strains. One was harbouring only GABA transaminase gene, and the other had both acdS and gabT enzymes. The acdS degrade ACC which is precursor of ethylene. Therefore, the strain also reduce ethylene during co-culticvation. These strains are increase the transformation frequency in tomato and erianthus.
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