Identification of essential elements for the plasmid transfer between different bacteria
| Project/Area Number |
24780087
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Shizuoka University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | プラスミド / 接合伝達 / 核様態タンパク質 |
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| Outline of Final Research Achievements |
The objective of this study is to find essential elements for the conjugative transfer of plasmid between different bacteria using pCAR1. Three kinds of nucleotide-associated proteins (NAPs) encoded on pCAR1 (Pmr, Phu, and Pnd) were shown to be important elements for its replication, maintenance and transfer in different host strains. Products of ORF145 and ORF146 on pCAR1 were not essential for the transfer but they may affect the transfer frequency of the plasmid. NAP genes were shown to be distributed on various transmissible plasmids. We successfully developed a method to detect, separate, and analyze ‘transient’ transconjugants, which would be important for the spread of plasmids in different host strains.
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Report
(4 results)
Research Products
(31 results)
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[Journal Article] Effects of three different nucleoid-associated proteins encoded on IncP-7 plasmid pCAR1 on host Pseudomonas putida KT24402015
Author(s)
Suzuki-Minakuchi C., R. Hirotani, M. Shintani, T. Takeda, Y. Takahashi, K. Matsui, D. Vasileva, C.-S. Yun, K. Okada, H. Yamane, and H. Nojiri
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Journal Title
Applied and Environmental Microbiology
Volume: 81
Issue: 8
Pages: 2869-2880
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Modulation of primary cell functions of host Pseudomonas bacteria by conjugative plasmid pCAR12015
Author(s)
Takahashi, Y., M. Shintani, N. Takase, Y. Kazou, F. Kawamura, H. Hara, H. Nishida, K. Okada, H. Yamane, and H. Nojiri
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Journal Title
Environmental Microbiology
Volume: 17
Issue: 1
Pages: 134-155
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Single-cell analyses revealed transfer ranges of IncP-1, IncP-7, and IncP-9 plasmids in a soil bacterial community2014
Author(s)
Shintani M, Matsui K, Inoue J, Hosoyama A, Ohji S, Yamazoe A, Nojiri H, Kinbara K, Ohkuma M
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Journal Title
Appl. Environ. Microbiol.
Volume: 80
Issue: 1
Pages: 138-145
DOI
Related Report
Peer Reviewed
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