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33S solid-state NMR analysis of methionine radical inducing cytotoxicity of amyloid-beta protein

Research Project

Project/Area Number 24780108
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionTohoku University

Principal Investigator

MASUDA YUICHI  東北大学, 薬学研究科(研究院), 助教 (90617755)

Co-Investigator(Renkei-kenkyūsha) TAKEGOSHI Kiyonori  京都大学, 大学院理学研究科, 教授 (10206964)
MURAKAMI Kazuma  京都大学, 大学院農学研究科, 准教授 (80571281)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords有機化学 / アミロイド / 固体NMR / 硫黄 / ラジカル
Research Abstract

We labeled sulfur atoms of methionine, cysteine and amyloid-beta with 33S by organic synthesis. We detected 33S NMR signals of methionine in solid-state and methionine sulfoxide in solution.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (31 results)

All 2014 2013 2012 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (20 results) Book (1 results) Remarks (3 results)

  • [Journal Article] Total Synthesis and Conformational Analysis of Apratoxin C2014

    • Author(s)
      Yuichi Masuda, Jun Suzuki, Masahito Yoshida, and Takayuki Doi
    • Journal Title

      Peptide Science 2013

      Volume: 巻番号なし Pages: 61-62

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of Photoaffinity Probe of Beauveriolides for Binding-site Analysis2014

    • Author(s)
      Yuichi Masuda, Kazumasa Aoyama, Masahito Yoshida, Keisuke Kobayashi, Nobuhiro Koyama, Hiroshi Tomoda, and Takayuki Doi
    • Journal Title

      Peptide Science 2013

      Volume: 巻番号なし Pages: 149-150

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Exploring various modulation-sideband recoupling conditions of SHA+ sequence at fast MAS.2013

    • Author(s)
      Shen, M., Liu, Q., Trebosc, J., Lafon, O., Masuda, Y., Takegoshi, K., Amoureux, J. P., Hu, B., Chen, Q.
    • Journal Title

      Solid State Nucl. Magn. Reson.

      Volume: 55-56 Pages: 42-47

    • DOI

      10.1016/j.ssnmr.2013.07.001

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Site-specific inhibitory mechanism for amyloid-β42 aggregation by catechol-type flavonoids targeting the Lys-residues2013

    • Author(s)
      Sato, M., Murakami, K., Ikubo, H., Uno, M., Nakagawa, Y., Katayama, S., Akagi, K.-i., Masuda, Y., Takegoshi, K., *Irie, K
    • Journal Title

      J.Biol.Chem

      Volume: 288 Issue: 32 Pages: 23212-23224

    • DOI

      10.1074/jbc.m113.464222

    • NAID

      120005477416

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Solid-state NMR analysis of the beta-strand orientation of the protofibrils of amyloid beta-protein2012

    • Author(s)
      Takashi Doi
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 428 Issue: 4 Pages: 458-462

    • DOI

      10.1016/j.bbrc.2012.10.096

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Very-long-distance correlations in proteins revealed by solid-state NMR spectroscopy2012

    • Author(s)
      Hu, B., Trebosc, J., Lafon, O., Chen, Q., Masuda, Y., Takegoshi, K., Amoureux, J. P.
    • Journal Title

      ChemPhysChem

      Volume: 13 Issue: 16 Pages: 3585-3588

    • DOI

      10.1002/cphc.201200548

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Lack of the Consensus Sequence Necessary for Tryptophan Prenylation in the ComX Pheromone Precursor2012

    • Author(s)
      F. Tsuji, A. Ishihara, A. Nakagawa, M. Okada, S. Kitamura, K. Kanamaru, Y. Masuda, K. Murakami, K. Irie, and Y. Sakagami.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 76 Issue: 8 Pages: 1492-1496

    • DOI

      10.1271/bbb.120206

    • NAID

      10031065914

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] アプラトキシンCの活性コンホメーションに着目した構造活性相関研究2014

    • Author(s)
      増田裕一,鈴木淳,吉田将人,土井隆行
    • Organizer
      日本薬学会第134年会
    • Place of Presentation
      熊本大学黒髪キャンパス,熊本
    • Related Report
      2013 Annual Research Report
  • [Presentation] 生物活性環状ペプチドPF1171類の固相法を用いた全合成2014

    • Author(s)
      増田裕一,田中錬,甲斐建次,A. Ganesan,土井隆行
    • Organizer
      日本薬学会第134年会
    • Place of Presentation
      熊本大学黒髪キャンパス,熊本
    • Related Report
      2013 Annual Research Report
  • [Presentation] タンパク質の高感度^<33>S-NMR解析に向けた^<33>S標識アミノ酸の合成2013

    • Author(s)
      増田裕一,松尾真嗣,水野敬,野田泰斗,竹腰清乃理,土井隆行
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      横浜(口頭発表, 講演番号29N-am09)
    • Year and Date
      2013-03-29
    • Related Report
      2013 Final Research Report
  • [Presentation] ^<33>S標識によるタンパク質の高感度33S-NMR解析に向けて2013

    • Author(s)
      増田裕一,松尾真嗣,水野敬,野田泰斗,竹腰清乃理,土井隆行
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      仙台(口頭発表, 講演番号4C26a03)
    • Year and Date
      2013-03-27
    • Related Report
      2013 Final Research Report
  • [Presentation] Synthesis of Photoaffinity-Labeled Beauveriolides for Binding-Site Analysis2013

    • Author(s)
      Yuichi Masuda, Kazumasa Aoyama, Masahito Yoshida, Keisuke Kobayashi, Nobuhiro Koyama, Hiroshi Tomoda, and Takayuki Doi
    • Organizer
      International Symposium for the 70th Anniversary of the Tohoku Branch of the Chemical Society of Japan
    • Place of Presentation
      Tohoku Univ, Sendai, Japan
    • Related Report
      2013 Annual Research Report
  • [Presentation] タンパク質間相互作用阻害機構の解明に向けたスピンラベル化Thielocin B1 の合成研究2013

    • Author(s)
      大澤宏祐,吉田将人,増田裕一,土井隆行
    • Organizer
      第52回日本薬学会東北支部大会
    • Place of Presentation
      東北大学,仙台
    • Related Report
      2013 Annual Research Report
  • [Presentation] 結合部位解明を指向した光反応性官能基を有するBeauveriolide誘導体の合成研究2013

    • Author(s)
      増田裕一,青山和賢,吉田将人,小林啓介、小山信裕,供田洋,土井隆行
    • Organizer
      第52回日本薬学会東北支部大会
    • Place of Presentation
      東北大学,仙台
    • Related Report
      2013 Annual Research Report
  • [Presentation] Total Synthesis and Conformational Analysis of Apratoxin C2013

    • Author(s)
      Yuichi Masuda, Jun Suzuki, Masahito Yoshida, and Takayuki Doi
    • Organizer
      APIPS 2013: 4th Asia-Pacific International Peptide Symposium, 50th Japanese Peptide Symposium
    • Place of Presentation
      Hotel Hankyu Expo Park, Osaka
    • Related Report
      2013 Annual Research Report
  • [Presentation] Synthesis of Photoaffinity Probe of Beauveriolides for Binding-site Analysis2013

    • Author(s)
      Yuichi Masuda, Kazumasa Aoyama, Masahito Yoshida, Keisuke Kobayashi, Nobuhiro Koyama, Hiroshi Tomoda, and Takayuki Doi
    • Organizer
      APIPS 2013: 4th Asia-Pacific International Peptide Symposium, 50th Japanese Peptide Symposium
    • Place of Presentation
      Hotel Hankyu Expo Park, Osaka
    • Related Report
      2013 Annual Research Report
  • [Presentation] 33S標識によるタンパク質の高感度33S-NMR解析に向けて2013

    • Author(s)
      増田裕一,松尾真嗣,水野敬,野田泰斗,竹腰清乃理,土井隆行
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      東北大学
    • Related Report
      2012 Research-status Report
  • [Presentation] タンパク質の高感度33S-NMR解析に向けた33S標識アミノ酸の合成2013

    • Author(s)
      増田裕一,松尾真嗣,水野敬,野田泰斗,竹腰清乃理,土井隆行
    • Organizer
      日本薬学会第133年会,
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] Solid-state NMR analysis of interaction sites of curcumin and amyloidβ-protein fibrils2012

    • Author(s)
      Masuda, Y., Fukuchi, M., Yatagawa, T., Tada, M., Takeda, K., Irie, K., Akagi, K., Monobe, Y., Imazawa, Y., Takegoshi, K.
    • Organizer
      IKCOC-12
    • Place of Presentation
      Kyoto(口頭発表, Program Number OP-21)
    • Year and Date
      2012-11-14
    • Related Report
      2013 Final Research Report
  • [Presentation] Specific Inhibitory Mechanism of Aggregation of 42-mer Amyloid-βPeptide by Flavonoids2012

    • Author(s)
      Sato, M., Murakami, K., Ikubo, H., Uno, M., Nakagawa, Y., Katayama, S., Akagi, K., Masuda, Y., Takegoshi, K., Irie, K.
    • Organizer
      IKCOC-12
    • Place of Presentation
      Kyoto(ポスター発表, Poster Number PA-140)
    • Year and Date
      2012-11-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Cryocoil MASプローブによる固体有機物の^<33>S NMR測定にむけて2012

    • Author(s)
      松尾真嗣,水野敬,野田泰斗,増田裕一,竹腰清乃理
    • Organizer
      第51回NMR討論会
    • Place of Presentation
      愛知(ポスター発表,講演番号P-81,査読なし)
    • Year and Date
      2012-11-10
    • Related Report
      2013 Final Research Report
  • [Presentation] カテコール系フラボノイド類によるアミロイドβの凝集抑制機構2012

    • Author(s)
      村上一馬,佐藤瑞穂,井久保遥子,宇野真弓,中川優,片山寿美枝,赤木謙一,増田裕一,竹腰清乃理,入江一浩
    • Organizer
      第54回天然有機化合物討論会
    • Place of Presentation
      東京(口頭発表,講演番号17,査読あり)
    • Year and Date
      2012-09-19
    • Related Report
      2013 Final Research Report
  • [Presentation] カテコール系フラボノイド類によるアミロイドβの凝集抑制機構2012

    • Author(s)
      村上一馬,佐藤瑞穂,井久保遥子,宇野真弓,中川 優,片山寿美枝,赤木謙一,増田裕一,竹腰清乃理,入江一浩
    • Organizer
      第54回天然有機化合物討論会
    • Place of Presentation
      東京農業大学 世田谷キャンパス
    • Related Report
      2012 Research-status Report
  • [Presentation] Solid-State NMR Analysis of Interaction Sites of Curcumin and Amyloid beta-Protein Fibrils2012

    • Author(s)
      Yuichi Masuda, Masashi Fukuchi, Tatsuya Yatagawa, Masato Tada, Kazuyuki Takeda, Kazuhiro Irie, Ken-ichi Akagi, Youko Monobe, Takayoshi Imazawa, K. Takegoshi
    • Organizer
      The 12th International Kyoto Conference on New Aspects of Organic Chemistry
    • Place of Presentation
      Rihga Royal Hotel Kyoto
    • Related Report
      2012 Research-status Report
  • [Presentation] Specific Inhibitory Mechanism of Aggregation of 42-mer Amyloid-beta Peptide by Flavonoids2012

    • Author(s)
      Mizuho Sato, Kazuma Murakami, Haruko Ikubo, Mayumi Uno, Yu Nakagawa, Sumie Katayama, Ken-ichi Akagi, Yuichi Masuda, Kiyonori Takegoshi, and Kazuhiro Irie
    • Organizer
      The 12th International Kyoto Conference on New Aspects of Organic Chemistry
    • Place of Presentation
      Rihga Royal Hotel Kyoto
    • Related Report
      2012 Research-status Report
  • [Presentation] コラーゲン分泌阻害剤の合成研究

    • Author(s)
      土井隆行,齋藤将純,伊藤進也,増田裕一,吉田将人,五島直樹,夏目徹,永田和宏
    • Organizer
      第51回日本薬学会東北支部大会
    • Place of Presentation
      青森大学
    • Related Report
      2012 Research-status Report
  • [Presentation] アプラトキシンCの合成研究

    • Author(s)
      土井隆行,鈴木淳,増田裕一,吉田将人
    • Organizer
      第51回日本薬学会東北支部大会
    • Place of Presentation
      青森大学
    • Related Report
      2012 Research-status Report
  • [Book] 遺伝子医学MOOK第21巻「最新ペプチド合成技術とその創薬研究への応用」(木曽良明編)2012

    • Author(s)
      村上一馬,増田裕一,入江一浩
    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://www.pharm.tohoku.ac.jp/~hannou/index.html

    • Related Report
      2013 Final Research Report
  • [Remarks] 東北大学薬学部 大学院薬学研究科 反応制御化学分野のホームページ

    • URL

      http://www.pharm.tohoku.ac.jp/~hannou/index.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] 東北大学薬学部大学院薬学研究科 反応制御化学分野のホームページ Doi Laboratory

    • URL

      http://www.pharm.tohoku.ac.jp/~hannou/index.html

    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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