Exploring novel paternally methylated imprinted genes using bi-maternal mouse fetuses
Project/Area Number |
24780265
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Applied animal science
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Research Institution | Hokkaido University |
Principal Investigator |
KAWAHARA manabu 北海道大学, (連合)農学研究科(研究院), 准教授 (70468700)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | 個体発生 / インプリント遺伝子 / 核移植 / 多型解析 / 卵子ゲノム / マウス / 哺乳類 / ゲノムインプリンティング / 胚発生 / 精子ゲノム / ゲノム刷り込み |
Outline of Final Research Achievements |
Parental genome functions in ontogeny are determined by interactions among transcripts from the maternal and paternal genomes. Comprehensive recognition of the interactions between parental genomes is important for understanding genomic imprinting in mammalian development. The bi-maternal (BM) embryos were constructed by only oocyte genomes, and therefore, analyses using those embryos enable us to investigate unknown paternally methylated imprinted genes in mouse genome. Comparing transcriptome between wild type (WT) and BM fetuses revealed that Mbnl1 expression in BM those was significantly reduced. We further examined Mbnl1 expression in uniparental fetuses, in which we found that Mbnl1 gene was downregulated in both parthenogenetic and androgenetic fetuses comparing that in WT fetus. These results suggested that Mbnl1 was non-imprinted gene but regulated by imprinted genes during development.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] Transcriptional wiring for establishing cell lineage specification at the blastocyst stage in cattle2013
Author(s)
Nagatomo, H., Kagawa, S., Kishi, Y., Takuma, T., Sada, A., Yamanaka, K., Abe, Y., Wada, Y., Takahashi, M., Kono, T., Kawahara, M.
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Journal Title
Biol. Reprod
Volume: Jun 27;88(6)
Issue: 6
Pages: 158-158
DOI
Related Report
Peer Reviewed
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[Journal Article] Dynamics of intracellular phospholipid membrane organization during oocyte maturation and successful vitrification of immature oocytes retrieved by ovum pick-up in cattle2013
Author(s)
Aono, A., Nagatomo, H., Takuma, T., Nonaka, R., Ono, Y., Wada, Y., Abe, Y., Watanabe, T., Kawahara, M.
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Journal Title
Theriogenology
Volume: 79
Issue: 8
Pages: 1146-1152
DOI
NAID
Related Report
Peer Reviewed
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