The investigation of the birth of methicillin-resistant Staphylococcus aureus (MRSA) by using genome-wide phylogenetic analysis.

• Project/Area Number: 24780298
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied veterinary science
• Research Institution: Rakuno Gakuen University
• Principal Investigator: TSUBAKISHITA SAE 酪農学園大学, 獣医学群, 准教授 (50616894)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: MRSA / whole-genome sequencing / Staphylococcus sciuri / 次世代シークエンサー / メチシリン耐性遺伝子 / mecA / S. fleurettii / SCCmec

Outline of Final Research Achievements

Methicillin-resistant Staphylococcus (S.) aureus (MRSA) is one of the most important multidrug-resistant pathogen of human and animals. The investigation of the birth of MRSA may contribute to more effective control measures for MRSA. In this study, to elucidate the birth of MRSA, we focus on the animal-borne staphylococci, which harbor the origin of the methicillin-resistant gene and its homologues, and performed the whole-genome sequencing and phylogenetic analysis of them. The results suggest the mobile genetic element encoding methicillin-resistance was composed on the chromosome of the animal-borne staphylococci before the element horizontally transferred to the chromosome of S. aureus.

Research Products

• [Journal Article] Genomic Basis for Methicillin Resistance in Staphylococcus aureus. (2013)
  • Author(s): Hiramatsu K, Ito T, Tsubakishita S, Sasaki T, Takeuchi F, Morimoto Y, Katayama Y, Matsuo M, Kuwahara-Arai K, Hishinuma T, Baba T.
  • Journal Title: Infect Chemother Volume: 45(2) Issue: 2 Pages: 117-136
  • DOI: 10.3947/ic.2013.45.2.117
  • Peer Reviewed
• [Journal Article] Population genetic structures of Staphylococcus aureus isolates from cats and dogs in Japan. (2012)
  • Author(s): Sasaki T, Tsubakishita S, Tanaka Y, Ohtsuka M, Hongo I, Fukata T, Kabeya H, Maruyama S, Hiramatsu K.
  • Journal Title: Journal of Clinical Microbiology Volume: 50(6) Issue: 6 Pages: 2152-2155
  • DOI: 10.1128/jcm.06739-11
  • Peer Reviewed
• [Presentation] Whole genome sequencing of members of the Staphylococcus sciuri group. (2014)
  • Author(s): Sae Tsubakishita
  • Organizer: International Union of Microbiological Societies 2014 Congress (IUMS)
  • Place of Presentation: モントリオール、カナダ
  • Year and Date: 2014-07-27 – 2014-08-01
• [Presentation] Whole genome sequencing of the animal-borne staphylococci as the reservoir of determinant of MRSA.
  • Author(s): 椿下 早絵
  • Organizer: 2012韓日微生物学シンポジウム
  • Place of Presentation: 扶余（韓国）
• [Book] Bacterial Integrative Mobile Genetic Elements CHAPTER18 Staphylococcal Cassette Chromosome (SCC): A Unique Gene Transfer System in Staphylococci (2013)
  • Author(s): Teruyo Ito, Sae Tsubakishita, Kyoko Kuwahara-Arai, Xiao Han, Keiichi Hiramatsu
  • Total Pages: 16
  • Publisher: Landes Bioscience