| Project/Area Number |
24790185
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Hokkaido University |
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Principal Investigator |
MIYAZAKI Taisuke 北海道大学, 医学(系)研究科(研究院), 助教 (90374230)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | プルキンエ細胞 / ルガロ細胞 / バスケット細胞 / 登上線維 |
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| Research Abstract |
In the developing cerebellum, climbing fiber (CF)-Purkinje cell (PC) synapses are eliminated from PC cell bodies, while the maturation of inhibitory interneurons such as basket (BC) or Lugaro cells (LC) proceeds. It has been unclear whether these excitatory and inhibitory inputs are directly correlated or not. In the present study, we investigated the process of CF-PC synapses elimination from PC cell bodies when the inhibitory interneurons are functionally disrupted. By analyses using with lentivirus and GFP-expressing reporter mouse, we found that somatic CF terminals were expelled by developing BC axons. In contrast to PC somata, CF terminals beneath PC layer increased in number during the second postnatal week, and CFs from multiple neuronal origins were found to innervate the somatodendritic domain of single LCs. Thus, the elimination of somatic CFs appears to proceed with the differentiation of inhibitory neuronal elements.
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