| Project/Area Number |
24790280
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Dokkyo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 細胞内シグナル伝達 / Wnt / リサイクリング / 上皮細胞 / 選別輸送 / クラスリン |
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| Research Abstract |
Selective basolateral secretion of Wnt3a in polarized epithelial cells was regulated by Wntless, clathrin and clathrin adaptor proteins. We found that Wntless was localized to the basolateral membrane. We also found that Wnt11 was sorted to the apical side and glycosylation was involved in the selective secretion of Wnt proteins. Furthermore, we found that a-taxilin, a syntaxin4 binding protein, was involved in the recycling of transferrin receptor. These results clarified a part of mechanism of selective secretion of Wnt in polarized epithelial cells and the function of regulator of intracellular vesicle traffic.
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