The role of exocytosis in biological functions

• Project/Area Number: 24790285
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: The University of Tokushima
• Principal Investigator: SAKANE Ayuko 徳島大学, ヘルスバイオサイエンス研究部, 助教 (60509777)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 高次生命機能システム / エキソサイトーシス / 細胞間接着形成 / 神経突起伸長・シナプス形成 / Rabファミリー低分子量G蛋白質

Research Abstract

Organization of individual cellular function as a whole system through receiving signals from extracellular environment and communicating with neighbors is vital for acquisition, expression, or maintenance of biological functions. Exocytosis, which is a one of main pathway of intracellular vesicular trafficking is taken as a pivotal factor to support the processes of the organization. In this study, we focus on the role of exocytosis in the formation of cell-cell contacts, neurite outgrowth, and the synapse formation leading to the organogenesis, or development and regeneration of neuronal system. We especially analyze the function of Rab family small G proteins and the related proteins and throw light on the molecular mechanisms of acquisition of biological functions.

Research Products

• [Journal Article] Roles of Rab family small G proteins in formation of the apical junctional complex in epithelial cells Cell Polarity (2014)
  • Author(s): Sakane A, and Sasaki T
  • Journal Title: Biological Role and Basic Mechanisms Volume: (in press)
• [Journal Article] Roles of Rab family small G proteins in formation of the apical junctional complex in epithelial cells (2014)
  • Author(s): Sakane, A. and Sasaki, T.
  • Journal Title: Cell Polarity: Biological Role and Basic Mechanisms Volume: 1
• [Journal Article] Junctional Rab13-binding protein (JRAB) re requires cell spreading via filamins. (2013)
  • Author(s): Sakane, A
  • Journal Title: Genes Cells Volume: 18 Issue: 9 Pages: 810-822
  • DOI: 10.1111/gtc.12078
  • Peer Reviewed
• [Journal Article] Rab13 small G protein and Junctional Rab13-binding protein (JRAB) orchestrate actin cytoskeletal organization during epithelial Junctional development (2012)
  • Author(s): Sakane, A., Abdallah, A.A.M., Nakano, K., Honda, K., Ikeda, W., Nishikawa, Y., Matsumoto, M., Matsushita, N., Kitamura. T., and Sasaki, T
  • Journal Title: J. Biol. Chem Volume: 287(51) Issue: 51 Pages: 42455-424680
  • DOI: 10.1074/jbc.m112.383653
  • Peer Reviewed
• [Journal Article] Expression of RASSF6 in kidney and the implication of RASSF6 and the Hippo pathway in the sorbitol-induced apoptosis in renal proximal tubular epithelial cells (2012)
  • Author(s): Withanage, K., Nakagawa, K., Ikeda, M., Kurihara, H., Kudo, T., Yang, Z., Sakane, A., Sasaki, T. and Hata, Y
  • Journal Title: J. Biochem Volume: 152(1) Issue: 1 Pages: 111-119
  • DOI: 10.1093/jb/mvs056
  • NAID: 10031166119
  • Peer Reviewed
• [Journal Article] Rab13 small G protein and Junctional Rab13-binding protein(JRAB) orchestrate actin cytoskeletal organization during epithelial junctional development. (2012)
  • Author(s): Sakane, A. et al.
  • Journal Title: J.Biol.Chem. Volume: 287 Pages: 42455-42468
  • Peer Reviewed
• [Journal Article] Expression of RASSF6 in kidney and the implication of RASSF6 and the Hippo pathway in the sorbitol-induced apoptosis in renal proximal tubular epithelial cells. (2012)
  • Author(s): Withanage, K. et al.
  • Journal Title: J. Biochem. Volume: 152 Pages: 111-119
  • Peer Reviewed
• [Presentation] 動く細胞 (2012)
  • Author(s): 坂根亜由子, 佐々木卓也
  • Organizer: ViEW2012ビジョン技術の実利用ワークショップ
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市)
  • Year and Date: 2012-12-06