| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Outline of Final Research Achievements |
In this study, we aimed to developed specific antibodies against resistant-factor for rapid detection of drug-resistant microorganisms. In this study, target molecules are key proteins of drug-resistance, 1) PBP2a of methicillin resistant Staphylococcus aureus (MRSA) and coagulase-negative staphylococci (MRCNS), 2) IMP-1 of carbapenem-resistant bacteria, and 3) oseltamivir- and peramivir-resistant type neuraminidase of influenza virus. I have prepared specific antibodies for them, and they showed the specificity with recombinant proteins and resistant/susceptible microorganisms. I constructed immunochromatography assay system using specific anti-PBP2a chicken IgY. It was revealed that it could detected MRSA and MRCNS directly from mimicked blood-cultured bottle in about 3 hours.
|
