Multiple analysis in colon of the stress-induced irritable bowel syndrome model
Project/Area Number |
24790582
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Pain science
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Research Institution | Hoshi University |
Principal Investigator |
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | 過敏性腸症候群 / 炎症 / サイトカイン / 下痢 / 消化管分泌 / CFTR / ケモカイン |
Outline of Final Research Achievements |
Diarrhea was induced by stress loaded with communication box in BALB/c mouse, although diarrhea was not caused in C57BL/6J mouse. Irritable bowel syndrome produces some symptoms similar to those of inflammatory bowel disease. In the present study, the gene expression of inflammatory cytokines in colon of the stress-loaded BALB/c mouse was examined. The expressions of various cytokines were attenuated by stress-loaded, although IL-4 and IL-17a gene expressions were increased by acute stress loaded, respectively. However, augmentation of these gene expressions was not correlated with development of diarrhea. On the other hand, gene expressions of TRPA1 and TRPV1 were increased by acute stress loaded. Furthermore, gene expression of cystic fibrosis transmembrane conductance regulator (CFTR) was increased by stress loaded. These results showed that the mechanism of stress-induced diarrhea is complicated in inflammation. Further studies are needed about involvement of TRPA1, TRPV1 and CFTR.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Possible involvement of activated locus coeruleus-noradrenergic neurons in pain-related sleep disorders.2015
Author(s)
Koh K, Hamada A, Hamada Y, Yanase M, Sakaki M, Someya K, Narita M, Kuzumaki N, Ikegami D, Sakai H, Iseki M, Inada E, Narita M.
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Journal Title
Neurosci Lett.
Volume: 589
Pages: 200-6
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Mechanisms of cisplatin-induced muscle atrophy.2014
Author(s)
Sakai H, Sagara A, Arakawa K, Sugiyama R, Hirosaki A, Takase K, Jo A, Sato K, Chiba Y, Yamazaki M, Matoba M, Narita M
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Journal Title
Toxicol Appl Pharmacol
Volume: 278
Issue: 2
Pages: 190-199
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Neutrophil recruitment is critical for 5-fluorouracil-induced diarrhea and the decrease in aquaporins in the colon2014
Author(s)
Sakai H, Sagara A, Matsumoto K, Jo A, Hirosaki A, Takase K, Sugiyama R, Sato K, Ikegami D, Horie S, Matoba M, Narita M.
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Journal Title
Pharmacol. Res.
Volume: 87
Pages: 71-79
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Changes in circadian rhythm for mRNA expression of melatonin 1A and 1B receptors in the hypothalamus under a neuropathic pain-like state.2014
Author(s)
Odo M, Koh K, Takada T, Yamashita A, Narita M, Kuzumaki N, Ikegami D, Sakai H, Iseki M, Inada E, Narita M.
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Journal Title
Synapse
Volume: 68
Issue: 4
Pages: 153-158
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Analysis of sleep disorders under pain using an optogenetic tool: possible involvement of the activation of dorsal raphe nucleus-serotonergic neurons.2013
Author(s)
Ito H, Yanase M, Yamashita A, Kitabatake C, Hamada A, Suhara Y, Narita M, Ikegami D, Sakai H, Yamazaki M, Narita M.
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Journal Title
Mol Brain
Volume: 6
Issue: 1
Pages: 59-59
DOI
Related Report
Peer Reviewed
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[Journal Article] Changes in the circadian rhythm of mRNA expression for µ-opioid receptors in the periaqueductal gray under a neuropathic pain-like state.2013
Author(s)
Takada T, Yamashita A, Date A, Yanase M, Suhara Y, Hamada A, Sakai H, Ikegami D, Iseki M, Inada E, Narita M.
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Journal Title
Synapse
Volume: 118
Issue: 5
Pages: 1076-1085
DOI
Related Report
Peer Reviewed
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