| Project/Area Number |
24790650
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Legal medicine
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| Research Institution | National Research Institute of Police Science |
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Principal Investigator |
WATANABE Ken 科学警察研究所, 法科学第一部, 研究員 (20532047)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Keywords | 物体検査 / 人体液斑の同定 / DNAメチル化 |
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| Research Abstract |
In this study, we analyzed aged stains of various body fluids by bisulfite sequence analysis for the methylation status in the previously reported semen-specific hypomethylated region, DACT1. The results indiate that the methylation status is stable in each old stain and that this can be a useful marker for forensic semen identification. We further developed a TaqMan realtime PCR detection method to make it easier to analyze the methylation status of DACT1 region. This method can be a useful supplementary tool for semen identification, especially from aged stains.
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