Xenotransplanted embryonic kidney provides a niche for endogenous stem cell differentiation into erythropoietin-producing tissue.
| Project/Area Number |
24790861
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Kidney internal medicine
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
Matsumoto Kei 東京慈恵会医科大学, 医学部, 助教 (30439799)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 腎性貧血 / 慢性腎不全 / エリスロポエチン / 再生医療 / 腎臓再生 / 腎性腎血 |
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| Outline of Final Research Achievements |
We established xenotransplantation models that control endogenous mesenchymal stem cell differentiation into mature erythropoietin (EPO)-producing tissue in a niche provided by a developing xenometanephros. Transplantation of rat metanephroi into mouse omentum, and similarly pig metanephroi into cat omentum, led to the recruitment of host cells and EPO production. EPO-expressing cells were not differentiated from integrating vessels because they did not co-express endothelial markers. Instead, EPO-expressing cells were shown to be derived from circulating host cells, as shown by EGFP expression in the grown transplants of chimeric mice bearing bone marrow from a transgenic(tg) mouse expressing EGFP under the control of the EPO promoter.These results suggest that bone marrow cells recruitment and differentiation in a xenotransplanted developing organ. Using metanephroi from tg suicide-inducible mice, the xeno-tissue component could be eliminated, leaving autologous EPO-producing tissue.
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Report
(5 results)
Research Products
(4 results)
