Development of novel fracture healing methods using growth factor containing a collagen-binding domain from Clostridium histolyticum collagenase
Project/Area Number |
24791560
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Orthopaedic surgery
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Research Institution | Kitasato University |
Principal Investigator |
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | コラーゲン結合型成長因子 / コラーゲン / 脱灰骨 / 骨折治癒 / コラーゲン結合ドメイン / 塩基性線維芽細胞増殖因子 / 成長因子 / 徐放 / 細菌学 / コラーゲン結合性成長因子 / 骨折治癒促進 |
Outline of Final Research Achievements |
We attempted to develop a collagen-based bone formation system using a fusion protein (collagen binding-bFGF, CB-bFGF) consisting of bFGF and the collagen-binding domain (CBD) of Clostridium histolyticum collagenase. The affinity of the fusion protein towards collagen and demineralized bone powder (DBP) was confirmed by collagen-binding assays. Moreover, the combination of the collagen binding-bFGF fusion protein (CB-bFGF) with collagen powder or DBP induced bone formation at protein concentrations lower than those required for bFGF alone in mice fracture models. Therefore, because of the potential adverse effects of bFGF, particularly when administered at high doses, the use of CP/CB-bFGF composite may be a safer alternative for bone repair by limiting bFGF exposure at fracture sites. Taken together, these properties suggest that the CB-bFGF/collagen powder composite is a promising injectable material for fracture healing in the clinical setting.
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] Acceleration of bone formation during fracture healing by injectable collagen powder and human basic fibroblast growth factor containing a collagen-binding domain from Clostridium histolyticum collagenase2013
Author(s)
Saito W, Uchida K, Ueno M, MatsushitaO, Inoue G, Nishi N, Ogura T, Hattori S, Fujimaki H, Tanaka K, Takaso M
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Journal Title
J Biomed Mater Res A
Volume: in press
Issue: 9
Pages: 3049-3055
DOI
Related Report
Peer Reviewed
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[Journal Article] Stability and reference interval of serum phosphoglucomutase (PGM)2012
Author(s)
Ikeya H, Oka S, Yukino S, Yano K, Suzuki H, Nishinari M, Uchida K, Naruse K, Kurosaki Y, Aoyama N, Ogawa Z.
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Journal Title
J Ana Bio-Sc
Volume: 35
Pages: 336-341
Related Report
Peer Reviewed
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