The Signaling Pathway of Lysophosphatidic Acid in Head and Neck Squamous Cell Carcinoma:The Role of Non-EDG Family Receptors
| Project/Area Number |
24791792
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Otorhinolaryngology
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| Research Institution | University of the Ryukyus |
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Principal Investigator |
MATAYOSHI Sen 琉球大学, 医学部附属病院, 助教 (60448587)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | リゾフォスファチジン酸 / LPA / 頭頸部癌 / Gタンパク共役受容体 / LPA4 / Gタンパク共役型受容体 |
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| Outline of Final Research Achievements |
Lysophosphatidic acids (LPAs) have been implicated in the tumorigenesis of head and neck squamous cell carcinoma (HNSCC). LPA receptors belong to endothelial differentiation gene (Edg) family (LPA1-3) and phylogenetically distant non-Edg family (LPA4- 6). In the present study, we investigated the effect of ectopic expression of LPA4 in SQ-20B, a HNSCC cell line, which expressed trivial level of endogenous LPA4. LPA stimulated proliferation of SQ-20B cells. Infection with doxycycline-regulatable adenovirus vector expressing green fluorescent protein-tagged LPA4 (AdvLPA4G) abolished LPA-stimulated proliferation in SQ-20B cells with the accumulation of G2/M-phasic cells. In the presence of Ki16425 or Rac1 inhibitor, ectopic LPA4-expressing SQ-20B cells showed further down-regulation of the proliferation. LPA induced cell motility was suppressed by ectopic LPA4 expression. Our data suggest that LPA4 signaling potentially modulates malignant behaviors of SQ-20B cells.
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Report
(4 results)
Research Products
(3 results)
