| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Lysophosphatidic acids (LPAs) have been implicated in the tumorigenesis of head and neck squamous cell carcinoma (HNSCC). LPA receptors belong to endothelial differentiation gene (Edg) family (LPA1-3) and phylogenetically distant non-Edg family (LPA4- 6). In the present study, we investigated the effect of ectopic expression of LPA4 in SQ-20B, a HNSCC cell line, which expressed trivial level of endogenous LPA4. LPA stimulated proliferation of SQ-20B cells. Infection with doxycycline-regulatable adenovirus vector expressing green fluorescent protein-tagged LPA4 (AdvLPA4G) abolished LPA-stimulated proliferation in SQ-20B cells with the accumulation of G2/M-phasic cells. In the presence of Ki16425 or Rac1 inhibitor, ectopic LPA4-expressing SQ-20B cells showed further down-regulation of the proliferation. LPA induced cell motility was suppressed by ectopic LPA4 expression. Our data suggest that LPA4 signaling potentially modulates malignant behaviors of SQ-20B cells.
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