Photodynamic therapy for intractable ulcer using blue LED
| Project/Area Number |
24791916
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plastic surgery
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| Research Institution | Osaka City University |
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Principal Investigator |
SHIRAKAWA Makiko 大阪市立大学, 医学(系)研究科(研究院), 医員 (30534630)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 光線力学療法 / 5-アミノレブリン酸 / MRSA / 5-ALA |
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| Research Abstract |
In this study, we evaluated the effectiveness of photodynamic therapy (PDT) using 5-aminolevulinic acid (5-ALA) and a 410-nm wavelength light emitting diode (LED) in vitro and in vivo for MRSA. We found that ALA-PDT with a 410-nm LED had an in vitro bactericidal effect on MRSA. An in vivo study, we demonstrated that protoporphyrin IX (PpIX) successfully accumulated in MRSA on the ulcer surface after intraperitoneal administration of 5-ALA to mice. Furthermore, we found that ALA-PDT accelerated wound healing and decreased the bacterial count on the ulcer surface. Our findings indicate that ALA-PDT may be a new treatment option for MRSA-infected wounds.
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Report
(3 results)
Research Products
(8 results)
