The effects of cell interaction between osteoclast precursor cell and T cell on osteoclast differentiation.

• Project/Area Number: 24792008
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Nihon University
• Principal Investigator: KAWATO Takayuki 日本大学, 歯学部, 准教授 (50386075)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: IL-18 / IL-18 binding protein / GM-CSF / RANKL / 破骨細胞 / T細胞 / RAW264.7 / CD4+ Tリンパ球

Outline of Final Research Achievements

It has been reported that IL-18 suppresses osteoclastogenesis by increasing GM-CSF production in T-cells. We examined the effect of RANKL-stimulated osteoclast precursors (RAW264.7 cells) on GM-CSF expression in IL-18-stimulated CD4+T-cells. RAW264.7 cells were induced into osteoclasts in the presence of RANKL. The production of IL-18 binding protein (BP) was increased in the culture medium derived from RANKL-stimulated RAW264.7 cells compared to unstimulated cells. GM-CSF expression in CD4+T-cells stimulated with IL-18 was suppressed by the addition of conditioned medium from RANKL-stimulated RAW264.7 cells. These results suggested that IL-18BP derived from RANKL-stimulated RAW264.7 cells blocks the stimulatory effects of IL-18 on GM-CSF expression in CT4+T-cells.

Research Products

• [Journal Article] Serum γ-glutamyltransferase level is associated with periodontal disease independent of drinking habits in Japanese adults. (2014)
  • Author(s): Morita T, Yamazaki Y, Fujiharu C, Ishii T, Seto M, Nishinoue N, Sasaki Y, Kawato T, Motohashi M, Maeno M
  • Journal Title: Medical Science Monitor Volume: 20 Pages: 2109-2016
  • DOI: 10.12659/msm.891204
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Angiotensin II suppresses osteoblastic differentiation and mineralized nodule formation via AT1 receptors in ROS17/2.8 cells. (2014)
  • Author(s): Nakai K, Kawato T, Morita T, Yamazaki Y, Tanaka H, Tonogi M, Oki H, Maeno M
  • Journal Title: Archives of Medical Science Volume: in press
  • Peer Reviewed
• [Journal Article] Angiotensin II induces the production of MMP-3 and MMP-13 through the MAPK signaling pathways via the AT1 receptor in osteoblasts. (2013)
  • Author(s): Nakai K, et al.
  • Journal Title: Biochimie. Volume: 95 Issue: 4 Pages: 922-933
  • DOI: 10.1016/j.biochi.2012.12.016
  • Peer Reviewed
• [Presentation] RANKL刺激したRAW264.7細胞はIL-18 binding protein 産生増加を介して CD4+T細胞のIL-18誘導性GM-CSF発現を抑制する
  • Author(s): 川戸貴行，北見 聡，田中秀樹，中井久美子，前野正夫
  • Organizer: 第22回硬組織再生生物学会・総会
  • Place of Presentation: 鶴見大学歯学部 (神奈川県)
• [Presentation] Angiotensin IIはAT1受容体 を介して骨芽細胞の分化と石灰化物形成を抑制する
  • Author(s): 中井久美子，川戸貴行，梶純也，原田修成，岡仁，富樫久美，柏木勝，前野正夫
  • Organizer: 第63回日本口腔衛生学会・総会
  • Place of Presentation: 熊本市民会館 崇城大学ホール (熊本県)
• [Presentation] アンジオテンシンIIはMAPKシグナル伝達経路を介して 骨芽細胞のMMP-13とMMP-3の産生を促進する
  • Author(s): 中井久美子
  • Organizer: 第62回日本口腔衛生学会 総会
  • Place of Presentation: キッセイ文化ホール (長野)