Investigation of diabetes-associated periodontitis pathogenesis from a point of relevance for advanced glycation end-product and redox regulation.

• Project/Area Number: 24792333
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Periodontal dentistry
• Research Institution: The University of Tokushima
• Principal Investigator: BANDO Mika 徳島大学, 大学病院, 助教 (10510000)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 終末糖化産物 / 歯肉線維芽細胞 / 酸化ストレス

Research Abstract

The aim of this study is investigation of diabetes-associated periodontitis pathogenesis from a point of oxidative stress, and utilization of antioxidative stress mechanism as new treatment. Advanced glycation end-product (AGE) and periodontopathic factor (P-LPS) increased expressions of AGE receptor, oxidative stress markers, antioxidative enzyme and inflammatory cytokine in human gingival fibroblasts. Inhibitors of MAPK pathway inhibited AGE-induced cytokine expression. KGF increased expression of antioxidative enzyme . These findigs suggest that AGE and P-LPS make a diabetes-associated periodontitis pathogenesis worse by production oxidative stress and inflammatory cytokine. KGF may enhance antioxidative stress mechanism.

Research Products

• [Journal Article] Mechanism of interleukin-1α transcriptional regulation of S100A9 in a human epidermal keratinocyte cell line (2013)
  • Author(s): Bando M, Zou X, Hiroshima Y, Kataoka M, K.F.Ross, Shinohara Y, Nagata T, M.C.Herzberg, Kido J
  • Journal Title: Biochimica et Biophysica Acta Volume: 1829 Pages: 954-962
  • Peer Reviewed
• [Journal Article] Simultaneous immunoassay analysis of plasma IL-6 and TNF-α on a microchip (2013)
  • Author(s): Abe K, Hashimoto Y, Yatsushiro S, Yamamura S, Bando M, Hiroshima Y, Kido J, Tanaka M, Shinohara Y, Ooie T, Baba Y, Kataoka M
  • Journal Title: PLOS ONE Volume: 8 Pages: 1-8
  • Peer Reviewed
• [Journal Article] Resistin in gingival crevecular fluid and induction of resistin release by Porphyromonas gingivalis lipopolysaccharide in human neutrophils (2012)
  • Author(s): Hiroshima Y, Bando M, Inagaki Y, Mihara C, Kataoka M, Murata H, Shinohara Y, Nagata T, Kido J
  • Journal Title: Journal of Periodontal Research Volume: 47 Pages: 554-562
  • Peer Reviewed
• [Journal Article] Gene polymorphism of β -defensin-1 is associated with susceptibility to periodontitis in Japanese
  • Author(s): Ikuta T, Inagaki Y, Tanaka K, Saito T, Nakajima Y, Bando M, Kido J, Nagata T
  • Journal Title: Odontology Volume: (in press)
  • Peer Reviewed
• [Presentation] 最終糖化産物は歯周組織細胞での炎症関連因子と酸化ストレス因子の発現に影響する (2014)
  • Author(s): 板東美香, 中島由紀子, 梶浦由加里, 木戸淳一, 稲垣裕司, 村田裕美, 永田俊彦
  • Organizer: 第57回日本糖尿病学会
  • Place of Presentation: リーガロイヤルホテル(大阪府)
  • Year and Date: 2014-05-22
• [Presentation] Effect of hypoxia and P. gingivalis - lipopolysaccharide on the expression of inflammation-related molecules in human oral keratinocytes (2014)
  • Author(s): Nakajima Y, Kido J, Bando M, Inagaki Y, Nagata T
  • Organizer: 第57回春季日本歯周病学会
  • Place of Presentation: 長良川国際会議場(岐阜県)
• [Presentation] 歯肉溝滲出液中グリコアルブミンおよびカルプロテクチンを指標とした糖尿病関連歯周炎の診断 (2014)
  • Author(s): 梶浦由加里, 板東美香, 木戸淳一, 稲垣裕司, 生田貴久, 橋本万里, 篠原宏貴, 二宮雅美, 村田裕美, 中島由紀子, 米田哲, 美原智恵, 廣島佑香, 大石慶二, 永田俊彦
  • Organizer: 第57回春季日本歯周病学会
  • Place of Presentation: 長良川国際会議場(岐阜県)
• [Presentation] 最終糖化産物は歯周組織細胞での炎症関連因子と酸化ストレス因子の発現に影響する (2014)
  • Author(s): 板東美香、中島由紀子、梶浦由加里、木戸淳一、稲垣裕司、村田裕美、永田俊彦
  • Organizer: 第57回日本糖尿病学会年次学術集会
  • Place of Presentation: リーガロイヤルホテル（大阪府北区）
• [Presentation] 最終糖化産物が歯肉線維芽細胞における酸化ストレス反応に及ぼす影響 (2013)
  • Author(s): 梶浦由加里, 板東美香, 木戸淳一, 稲垣裕司, 永田俊彦
  • Organizer: 第56回春季日本歯周病学会
  • Place of Presentation: タワーホール船堀(東京都)
  • Year and Date: 2013-06-01
• [Presentation] Advanced glycation end-product increases oxidative stress in human gingival fibroblasts (2013)
  • Author(s): Kajiura Y, Bando M, Inagaki Y, Murata H, Kido J, Nagata T
  • Organizer: Korean Academy of Periodontology Meeting
  • Place of Presentation: Grand Hilton Hotel (Seoul, Korea)
• [Presentation] Diagnosis of diabetes-associated periodontitis using glycoalbumin and calprotectin in gingival crevicular fluid (2013)
  • Author(s): Kido J, Bando M, Inagaki Y, Hiroshima Y, Murata H, Mihara C, Kajiura Y, Ikuta T, Shinohara H, Hashimoto M, Nakajima Y, Bando Y, Funaki M, Saito H, Nagata T
  • Organizer: 10th Asian Pacific Society of Periodontology Meeting
  • Place of Presentation: 奈良県新公会堂(奈良県)
• [Presentation] 糖尿病関連歯周炎の診断マーカーとしての歯肉溝滲出液中グリコアルブミンとカルプロテクチンの有用性 (2013)
  • Author(s): 木戸淳一, 板東美香, 坂東由記子, 稲垣裕司, 廣島佑香, 梶浦由加里, 美原智恵, 村田裕美, 生田貴久, 篠原宏貴, 橋本万里, 船木真理, 齋藤晴比古, 永田俊彦
  • Organizer: 第56回日本糖尿病学会
  • Place of Presentation: ホテル日航熊本(熊本県)
• [Presentation] Advanced Glycation End-product Induces Oxidative Stress in Human Gingival Fibroblasts (2013)
  • Author(s): 梶浦由加里、板東美香、稲垣裕司、村田裕美、木戸淳一、永田俊彦
  • Organizer: The 53rd General Session of Korean Academy of Periodontology
  • Place of Presentation: Grand Hilton Hotel (Korea Seoul)
• [Presentation] 最終糖化産物が歯肉線維芽細胞における酸化ストレス反応に及ぼす影響 (2013)
  • Author(s): 梶浦由加里
  • Organizer: 第56回春季日本歯周病学会
  • Place of Presentation: タワーホール船堀（東京都）
• [Presentation] Lipopolysaccharide induces oxidative stress and antioxidant responses in gingival fibroblasts (2012)
  • Author(s): 板東美香
  • Organizer: 第60回国際歯科研究学会日本部会(JADR)学術大会
  • Place of Presentation: 新潟コンベンションセンター（新潟県）