Investigation on coordinate regulation of reproduction and related behaviors by kisspeptin neuronal system
| Project/Area Number |
24870007
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Animal physiology/Animal behavior
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KANDA Shinji 東京大学, 理学(系)研究科(研究院), 助教 (50634284)
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | キスペプチン / in situ hybridization / パッチクランプ / トランスジェニック / ノックアウト / 神経内分泌 / 神経ペプチド / メダカ / イソトシン / バソトシン / 電気生理 |
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| Research Abstract |
In this study, to unveil the novel neuronal circuits that are regulated by kisspeptin neurons in vertebrates, I visualized kisspeptin receptor-expressing neurons with GFP, and performed RNAseq of GFP positive neurons in medaka. It was shown that the peptidergic neurons that are suggested to be involved in feeding behavior and homeostasis are expressing kisspeptin receptor. These suggestion were finally confirmed by double labeling of GFP and mRNA of a certain neurotransmitter by in situ hybridization. Following electrophysiological examination showed that kisspepitn synthetic peptide depolarize these kisspeptin receptor expressing neurons. As peptidergic neurons such as isotocin and vasotocin neurons were shown to express kisspeptin receptor, knockout medaka lines of these transmitters by using TALEN technology. Further examination of these neural circuits will be examined by more detailed electrophysiology utilizing the generated knockout and transgenic medaka lines.
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Report
(3 results)
Research Products
(53 results)
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[Journal Article] Whole brain-pituitary in vitro preparation of the transgenic medaka (Oryzias latipes) as a tool for analyzing the differential regulatory mechanisms of LH and FSH release2014
Author(s)
Karigo, T., Aikawa, M., Kondo, C., Abe, H., Kanda, S. and Oka, Y.
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Journal Title
Endocrinology
Volume: Vol. 155
Pages: 536-547
Related Report
Peer Reviewed
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[Presentation] Electrophysiological and morphological analysis of Kiss1 neurons in trasgenic medaka, Oryzias lapes2012
Author(s)
Shimada, H., Kanda, S., Akazome, Y., Abe, H., Okubo, K., Oka, Y.
Organizer
2nd world congress of kisspeptin
Place of Presentation
Tokyo
Related Report
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[Presentation] Embryonic expression and function of kisspeptins and their receptors in a teleost fish, medaka2012
Author(s)
Okubo, K., Hodne, K., Kanda, S., Shimada, H., Oka, Y., Welzien, F.A.
Organizer
2nd world congress of kisspeptin
Place of Presentation
Tokyo
Related Report
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[Presentation] Effects of kisspeptins on LH secretion and GnRH1 neuronal activities in goldfish and medaka2012
Author(s)
Karigo, T., Uenoyama, Y., Oishi, S., Fujii, N., Mitani, Y., Kanda, S., Oka, Y.
Organizer
2nd world congress of kisspeptin
Place of Presentation
Tokyo
Related Report
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