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Accurate quantification of wood biomass and analyses during its biodegradation

Research Project

Project/Area Number 24880022
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Wood science
Research InstitutionKyoto University

Principal Investigator

NISHIMURA Hiroshi  京都大学, 生存圏研究所, 助教 (50553989)

Project Period (FY) 2012-08-31 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsバイオマス / NMR / リグニン / 担子菌 / 木材
Outline of Final Research Achievements

Accurate and quantified analysis is needed to understand the biological degradation of wood biomass at a molecular level. In order to estimate quantitatively in the biomass sample containing complicated and various molecules, we have developed methodology to correct errors occurring in the 2D-HSQC NMR measurements. The variation in the signal integration value originating in the relaxation process of each molecule and differences of a spin couple state were . We also measured the dynamic change of extracellular metabolites and the structural change of a cell wall molecules in a wood decay.

Report

(3 results)
  • 2014 Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (11 results)

All 2014 Other

All Presentation (11 results)

  • [Presentation] Development of new methods to compensate distortion of quantitation caused by difference in molecular weight (transverse relaxation time)2014

    • Author(s)
      Okamura, H., Nishimura, H., Nagata, T., Kigawa, T., Watanabe, T., Katahira, M.
    • Organizer
      53rd Ann. Meeting NMR Soc. Japan
    • Place of Presentation
      Osaka
    • Year and Date
      2014-11-04 – 2014-11-06
    • Related Report
      2013 Annual Research Report
  • [Presentation] Structural analysis of lignin-carbohydrate complex.2014

    • Author(s)
      Nishimura H., Kamiya A., Katahira M., WatanabeT.
    • Organizer
      3rd Symp. Biotechnol. Appl. Lignocelluloses
    • Place of Presentation
      Concepcion CHILE
    • Year and Date
      2014-10-27
    • Related Report
      2013 Annual Research Report
  • [Presentation] Structural analyses of lignin using ultra-high sensitivity NMR for biorefinery2014

    • Author(s)
      Watanabe, T., Nishimura, H., Okamura, H., Sakai, H., Nakamura, M., Nakamura, H., Takaya, M., Katahira, M.
    • Organizer
      5th Int. Symp. Adv. Energy Sci.
    • Place of Presentation
      Kyoto
    • Year and Date
      2014-09-30 – 2014-10-02
    • Related Report
      2013 Annual Research Report
  • [Presentation] Development of new NMR methods for correct quantitation on biomass components2014

    • Author(s)
      Okamura, H., Nishimura, H., Nagata, T., KIgawa, T., Watanabe, T., Katahira, M.
    • Organizer
      5th Int. Symp. Adv. Energy Sci.
    • Place of Presentation
      Kyoto
    • Year and Date
      2014-09-30 – 2014-10-02
    • Related Report
      2013 Annual Research Report
  • [Presentation] 木材腐朽菌によるリグニン分解過程の解析2014

    • Author(s)
      西村裕志, 酒井洋尚, 片平正人, 渡辺隆司
    • Organizer
      第59回リグニン討論会
    • Place of Presentation
      福井
    • Year and Date
      2014-09-12
    • Related Report
      2013 Annual Research Report
  • [Presentation] NMR study of Lignin-carbohydrate Linkage in Milled Wood2014

    • Author(s)
      Nishimura H., Kamiya A., Katahira M., WatanabeT.
    • Organizer
      Polyphenols Communications 2014, 331-332
    • Place of Presentation
      Nagoya
    • Year and Date
      2014-09-02 – 2014-09-06
    • Related Report
      2013 Annual Research Report
  • [Presentation] 木質バイオマスの生分解機構の解析2014

    • Author(s)
      西村裕志
    • Organizer
      第259回生存圏シンポジウム 第5回DASH/FBAS成果報告会
    • Place of Presentation
      京都
    • Year and Date
      2014-06-16
    • Related Report
      2013 Annual Research Report
  • [Presentation] Analysis of wood cell wall structures and secondary metabolites during the biodegradation of white-rot fungi using solution NMR

    • Author(s)
      Hiroshi Nishimura, Masato Katahira, Takashi Watanabe
    • Organizer
      Lignobiotech II, (Plenary Lecture)
    • Place of Presentation
      ACROS Fukuoka, Japan
    • Related Report
      2012 Annual Research Report
  • [Presentation] High resolution and quantitative NMR analysis of whole milled wood and biodegraded wood

    • Author(s)
      Hiroshi Nishimura
    • Organizer
      International Symposium on Sustainable Development and Human Security in Southeast Asia through Biorefinery and Low Cost House(SABH2012):59-62
    • Place of Presentation
      Kyoto Univ., Uji, Japan
    • Related Report
      2012 Annual Research Report
  • [Presentation] Biodegradation of Wood Biomass by Selective White-rot Fungi

    • Author(s)
      Hiroshi Nishimura
    • Organizer
      The first Bristol-Kyoto Symposium 2013
    • Place of Presentation
      Bristol, England
    • Related Report
      2012 Annual Research Report
  • [Presentation] NMR法によるリグノセルロースの微生物分解の解析

    • Author(s)
      西村 裕志, 田頭 英朗, 岡村 英保, 小澤 佑, 片平 正人, 渡辺 隆司
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東北大学, 仙台
    • Related Report
      2012 Annual Research Report

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Published: 2012-11-27   Modified: 2019-07-29  

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