Identification of novel host factors responsible for the functional regulation of influenza virus ribonucleoprotein complexes

• Project/Area Number: 24890029
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: University of Tsukuba
• Principal Investigator: KAWAGUCHI Atsushi 筑波大学, 医学医療系, 助教 (90532060)
• Project Period (FY): 2012-08-31 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: インフルエンザウイルス / 宿主因子 / リボヌクレオプロテイン / 微小管 / 細胞内輸送 / Ribonucleoprotein / リサイクリングエンドソーム / 微小管合成中心

Research Abstract

The genome of influenza virus replicates in the nucleus. The replicated viral genome forms ribonucleoprotein (RNP) complexes. The progeny RNP complexes are exported to the cytoplasm and acculate in the microtubule-organizing center (MTOC) and then moves to the budding site beneath the cell surface along microtubules. However, the regulatory mechanism of replicational and post-replicational processes of the RNP are largely unknown. Here, we identified YB-1, which is a cellular RNA-binding protein, as a novel viral RNP binding protein. We found that YB-1 is a porter that leads viral RNP to MTOC. Further, we also examined enzymatic activities of viral polymerases purified from either high or low pathogenic strain using biochemical assays.

Research Products

• [Journal Article] Long Noncoding RNA NEAT1-Dependent SFPQ Relocation from Promoter Region to Paraspeckle Mediates IL8 Expression upon Immune Stimuli (2014)
  • Author(s): Imamura K, Imamachi N, Akizuki G, Kumakura M, Kawaguchi A, Nagata K, Kato A, Kawaguchi Y, Sato H, Yoneda M, Kai C, Yada T, Suzuki Y, Yamada T, Ozawa T, Kaneki K, Inoue T, Kobayashi M, Kodama T, Wada Y, Sekimizu K, Akimitsu N
  • Journal Title: Mol. Cell Volume: 53(3) Pages: 393-406
  • Peer Reviewed
• [Journal Article] Involvement of the N-terminal portion of influenza virus RNA polymerase subunit PB1 in nucleotide recognition (2014)
  • Author(s): Binh NT, Wakai C, Kawaguchi A, Nagata K
  • Journal Title: Biochem Biophys. Res. Commun Volume: 443(3) Pages: 975-979
  • NAID: 120007130532
  • Peer Reviewed
• [Journal Article] Long Noncoding RNA NEAT1-Dependent SFPQ Relocation from Promoter Region to Paraspeckle Mediates IL8 Expression upon Immune Stimuli (2014)
  • Author(s): Imamura K, Imamachi N, Akizuki G, Kumakura M, Kawaguchi A, Nagata K, Kato A, Kawaguchi Y, Sato H, Yoneda M, Kai C, Yada T, Suzuki Y, Yamada T, Ozawa T, Kaneki K, Inoue T, Kobayashi M, Kodama T, Wada Y, Sekimizu K and Akimitsu N.
  • Journal Title: Molecular cell Volume: 53 Issue: 3 Pages: 393
  • DOI: 10.1016/j.molcel.2014.01.009
  • Peer Reviewed / Open Access
• [Journal Article] Involvement of the N-terminal portion of influenza virus RNA polymerase subunit PB1 in nucleotide recognition. (2014)
  • Author(s): Binh NT, Wakai C, Kawaguchi A, Nagata K.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 443 Issue: 3 Pages: 975-979
  • DOI: 10.1016/j.bbrc.2013.12.071
  • NAID: 120007130532
  • Peer Reviewed
• [Journal Article] The N-terminal region of influenza virus polymerase PB1 adjacent to the PA binding site is involved in replication but not transcription of the viral genome (2013)
  • Author(s): Binh NT, Wakai C, Kawaguchi A, Nagata K
  • Journal Title: Front. Microbiol Volume: 4 Pages: 398-398
  • NAID: 120007130538
  • Peer Reviewed
• [Journal Article] The N-terminal region of influenza virus polymerase PB1 adjacent to the PA binding site is involved in replication but not transcription of the viral genome. (2013)
  • Author(s): Binh NT, Wakai C, Kawaguchi A, Nagata K.
  • Journal Title: Frontiers in Microbiology Volume: 4 Pages: 398-398
  • NAID: 120007130538
  • Peer Reviewed
• [Journal Article] Critical Role of an Antiviral Stress Granule Containing RIG-I and PKR in Viral Detection and Innate Immunity (2012)
  • Author(s): Onomoto K, Jogi M, Yoo J-S, Narita R, Morimoto S, Takemura A, Sambhara S, Kawaguchi A, Osari S, Nagata K, Matsumiya T, Namiki H, Yoneyama M, Fujita T
  • Journal Title: PLoS One Volume: 7 Issue: 8 Pages: 1-15
  • DOI: 10.1371/journal.pone.0043031
  • Peer Reviewed
• [Journal Article] YB-1 functions as a porter to lead influenza virus ribonucleoprotein complexes to microtubules (2012)
  • Author(s): Kawaguchi A, Matsumoto K, Nagata K
  • Journal Title: J. Virol Volume: 86(20) Pages: 11086-11095
  • NAID: 120007130530
  • Peer Reviewed
• [Journal Article] YB-1, Y-box binding protein-1, is a porter to lead influenza virus ribonucleoprotein complexes to microtubules. (2012)
  • Author(s): Kawaguchi, A., Matsumoto, K., and Nagata, K.
  • Journal Title: Journal of Virology Volume: 86 Issue: 20 Pages: 11086-11095
  • DOI: 10.1128/jvi.00453-12
  • Peer Reviewed
• [Presentation] ウイルスポリメラーゼによって規定されるトリインフルエンザウイルスの哺乳類細胞への適応機構 (2013)
  • Author(s): 原田芳美、川口敦史、永田恭介
  • Organizer: 平成25年度日本生化学会関東支部例会
  • Place of Presentation: 甲府
  • Year and Date: 2013-06-15
• [Presentation] ポリコーム複合体因子であるEZH2はインフルエンザウイルスタンパク質M1およびインフルエンザウイルスゲノムの核外輸送を制御する (2013)
  • Author(s): 浅賀正充、川口敦史、永田恭介
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] インフルエンザウイルス感染に応答した宿主因子YB-1による中心体の機能制御 (2013)
  • Author(s): 川口敦史、永田恭介
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] インフルエンザポリメラーゼ遺伝子間の適合性 (2013)
  • Author(s): 原田芳美、川口敦史、永田恭介
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] インフルエンザウイルスゲノムの細胞内輸送機構におけるNS1の新規機能 (2013)
  • Author(s): 長利卓、川口敦史、永田恭介
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] Harald Wodrich, Nagata K, Michael Kann. In cellulo analysis of parvovirus - mediated changes of nuclear envelope integrity by real time microscopy (2013)
  • Author(s): Kenza Snoussi, Kawaguchi A, Kato K
  • Organizer: Leading Graduate Schools International Conference (Tsukuba Global Science Week)
  • Place of Presentation: Tsukuba
• [Presentation] YB-1 is a porter to lead influenza virus ribonucleoprotein complexes to microtubules for the virus production (2013)
  • Author(s): Kawaguchi A
  • Organizer: The 2^<nd> Meeting on RNA and Biofunctions-Asia Study"RNA Biofunctions and Viruses"
  • Place of Presentation: Fukuoka
• [Presentation] In cellulo analysis of parvovirus-mediated changes of nuclear envelope integrity by real time microscopy. (2013)
  • Author(s): Kenza Snoussi, Kawaguchi A, Kato K, Harald Wodrich, Nagata K, Michael Kann.
  • Organizer: Leading Graduate Schools International Conference
  • Place of Presentation: つくば
• [Presentation] YB-1 is a porter to lead influenza virus ribonucleoprotein complexes to microtubules. (2013)
  • Author(s): Kawaguchi A, Nagata K.
  • Organizer: 15th International Negative Strand Virus Meeting 2013.
  • Place of Presentation: グラナダ（スペイン）
• [Presentation] YB-1 is a porter to lead influenza virus ribonucleoprotein complexes to microtubules for the virus production. (2013)
  • Author(s): Kawaguchi A.
  • Organizer: The 2nd Meeting on RNA and Bionfunctions-Asia Study "RNA Biofunctions and Viruses"
  • Place of Presentation: 福岡
• [Presentation] YB-1 is a porter to lead influenza virus ribonucleoprotein complexes to microtubules (2012)
  • Author(s): Kawaguchi A, Nagata K
  • Organizer: The 3rd Leading Graduate Schools International Conference (Tsukuba Global Science Week)
  • Place of Presentation: Tsukuba
• [Presentation] A novel function of NS1 viral protein in intracellular of the influenza virus genome (2012)
  • Author(s): Osari S, Kawaguchi A, Nagata K
  • Organizer: The 7th Tsukuba Medical Science Research Meeting (Tsukuba Global Science Week)
  • Place of Presentation: Tsukuba
• [Presentation] 新規宿主因子YB-1によるインフルエンザウイルスゲノムの細胞内輸送制御 (2012)
  • Author(s): 川口敦史、松本健、永田恭介
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
• [Presentation] 新規宿主因子YB-1によるインフルエンザウイルスRNP複合体の微小管への輸送機構解析 (2012)
  • Author(s): 川口敦史、松本健、永田恭介
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] EZH2はインフルエンザゲノムの核外輸送を制御する (2012)
  • Author(s): 浅賀正充、川口敦史、永田恭介
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] YB-1 is a porter to lead influenza virus ribonucleoprotein complexes to microtubules. (2012)
  • Author(s): Kawaguchi A.
  • Organizer: The 3rd Leading Graduate Schools International Conference
  • Place of Presentation: つくば
• [Presentation] A novel function of NS1 viral protein in intracellular of the influenza virus genome. (2012)
  • Author(s): Osari S.
  • Organizer: The 7th Tsukuba Medical Science Research Meeting
  • Place of Presentation: つくば
• [Presentation] 新規宿主因子YB-1によるインフルエンザウイルスRNP複合体の微小管への輸送機構解析 (2012)
  • Author(s): 川口敦史
  • Organizer: 第35回日本分子生物学会年回
  • Place of Presentation: 福岡
• [Presentation] EZH2はインフルエンザウイルスゲノムの核外輸送を制御する (2012)
  • Author(s): 浅賀正充
  • Organizer: 第35回日本分子生物学会年回
  • Place of Presentation: 福岡
• [Presentation] ウイルスポリメラーゼによって規定されるトリインフルエンザウイルスの哺乳類細胞への適応機構
  • Author(s): 原田芳美、 川口敦史、 永田恭介
  • Organizer: 平成25年度日本生化学会関東支部例会
  • Place of Presentation: 甲府
• [Remarks]
  • URL: http://www.md.tsukuba.ac.jp/basic-med/infectionbiology/virology/
• [Remarks] 川口研究室ホームページ
  • URL: http://www.md.tsukuba.ac.jp/basic-med/kawaguchilab/