Identification of novel host factors responsible for the functional regulation of influenza virus ribonucleoprotein complexes
| Project/Area Number |
24890029
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| Research Category |
Grant-in-Aid for Research Activity start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | インフルエンザウイルス / 宿主因子 / リボヌクレオプロテイン / 微小管 / 細胞内輸送 / Ribonucleoprotein / リサイクリングエンドソーム / 微小管合成中心 |
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| Research Abstract |
The genome of influenza virus replicates in the nucleus. The replicated viral genome forms ribonucleoprotein (RNP) complexes. The progeny RNP complexes are exported to the cytoplasm and acculate in the microtubule-organizing center (MTOC) and then moves to the budding site beneath the cell surface along microtubules. However, the regulatory mechanism of replicational and post-replicational processes of the RNP are largely unknown. Here, we identified YB-1, which is a cellular RNA-binding protein, as a novel viral RNP binding protein. We found that YB-1 is a porter that leads viral RNP to MTOC. Further, we also examined enzymatic activities of viral polymerases purified from either high or low pathogenic strain using biochemical assays.
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Report
(3 results)
Research Products
(32 results)
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[Journal Article] Long Noncoding RNA NEAT1-Dependent SFPQ Relocation from Promoter Region to Paraspeckle Mediates IL8 Expression upon Immune Stimuli2014
Author(s)
Imamura K, Imamachi N, Akizuki G, Kumakura M, Kawaguchi A, Nagata K, Kato A, Kawaguchi Y, Sato H, Yoneda M, Kai C, Yada T, Suzuki Y, Yamada T, Ozawa T, Kaneki K, Inoue T, Kobayashi M, Kodama T, Wada Y, Sekimizu K, Akimitsu N
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Journal Title
Mol. Cell
Volume: 53(3)
Pages: 393-406
Related Report
Peer Reviewed
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[Journal Article] Long noncoding RNA NEAT1-dependent SFPQ relocation from promoter region to paraspeckle mediates IL8 expression upon immune stimuli2014
Author(s)
Imamura K, Imamachi N, Akizuki G, Kumakura M, Kawaguchi A, Nagata K, Kato A, Kawaguchi Y, Sato H, Yoneda M, Kai C, Yada T, Suzuki Y, Yamada T, Ozawa T, Kaneki K, Inoue T, Kobayashi M, Kodama T, Wada Y, Sekimizu K and Akimitsu N.
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Journal Title
Mol. Cell
Volume: 53
Pages: 393-406
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Critical role of antiviral stress granule containing RIG-I and PKR in viral detection and innate immunity2012
Author(s)
Onomoto K, Jogi M, Yoo JS, Narita R, Morimoto S, Takemura A, Sambhara S, Kawaguchi A, Osari S, Nagata K, Matsumiya T, Namiki H, Yoneyama M, Fujita T
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Critical Role of an Antiviral Stress Granule Containing RIG-I and PKR in Viral Detection and Innate Immunity.2012
Author(s)
Onomoto K, Jogi M, Yoo J-S, Narita R, Morimoto S, Takemura A, Sambhara S, Kawaguchi A, Osari S, Nagata K, Matsumiya T, Namiki H, Yoneyama M, Fujita T
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Journal Title
DOI
Related Report
Peer Reviewed
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