• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Identification of novel host factors responsible for the functional regulation of influenza virus ribonucleoprotein complexes

Research Project

Project/Area Number 24890029
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Virology
Research InstitutionUniversity of Tsukuba

Principal Investigator

KAWAGUCHI Atsushi  筑波大学, 医学医療系, 助教 (90532060)

Project Period (FY) 2012-08-31 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsインフルエンザウイルス / 宿主因子 / リボヌクレオプロテイン / 微小管 / 細胞内輸送 / Ribonucleoprotein / リサイクリングエンドソーム / 微小管合成中心
Research Abstract

The genome of influenza virus replicates in the nucleus. The replicated viral genome forms ribonucleoprotein (RNP) complexes. The progeny RNP complexes are exported to the cytoplasm and acculate in the microtubule-organizing center (MTOC) and then moves to the budding site beneath the cell surface along microtubules. However, the regulatory mechanism of replicational and post-replicational processes of the RNP are largely unknown. Here, we identified YB-1, which is a cellular RNA-binding protein, as a novel viral RNP binding protein. We found that YB-1 is a porter that leads viral RNP to MTOC. Further, we also examined enzymatic activities of viral polymerases purified from either high or low pathogenic strain using biochemical assays.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • Research Products

    (31 results)

All 2014 2013 2012 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Open Access: 1 results) Presentation (20 results) Remarks (2 results)

  • [Journal Article] Long Noncoding RNA NEAT1-Dependent SFPQ Relocation from Promoter Region to Paraspeckle Mediates IL8 Expression upon Immune Stimuli2014

    • Author(s)
      Imamura K, Imamachi N, Akizuki G, Kumakura M, Kawaguchi A, Nagata K, Kato A, Kawaguchi Y, Sato H, Yoneda M, Kai C, Yada T, Suzuki Y, Yamada T, Ozawa T, Kaneki K, Inoue T, Kobayashi M, Kodama T, Wada Y, Sekimizu K, Akimitsu N
    • Journal Title

      Mol. Cell

      Volume: 53(3) Pages: 393-406

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of the N-terminal portion of influenza virus RNA polymerase subunit PB1 in nucleotide recognition2014

    • Author(s)
      Binh NT, Wakai C, Kawaguchi A, Nagata K
    • Journal Title

      Biochem Biophys. Res. Commun

      Volume: 443(3) Pages: 975-979

    • NAID

      120007130532

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Long Noncoding RNA NEAT1-Dependent SFPQ Relocation from Promoter Region to Paraspeckle Mediates IL8 Expression upon Immune Stimuli2014

    • Author(s)
      Imamura K, Imamachi N, Akizuki G, Kumakura M, Kawaguchi A, Nagata K, Kato A, Kawaguchi Y, Sato H, Yoneda M, Kai C, Yada T, Suzuki Y, Yamada T, Ozawa T, Kaneki K, Inoue T, Kobayashi M, Kodama T, Wada Y, Sekimizu K and Akimitsu N.
    • Journal Title

      Molecular cell

      Volume: 53 Issue: 3 Pages: 393

    • DOI

      10.1016/j.molcel.2014.01.009

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Involvement of the N-terminal portion of influenza virus RNA polymerase subunit PB1 in nucleotide recognition.2014

    • Author(s)
      Binh NT, Wakai C, Kawaguchi A, Nagata K.
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 443 Issue: 3 Pages: 975-979

    • DOI

      10.1016/j.bbrc.2013.12.071

    • NAID

      120007130532

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The N-terminal region of influenza virus polymerase PB1 adjacent to the PA binding site is involved in replication but not transcription of the viral genome2013

    • Author(s)
      Binh NT, Wakai C, Kawaguchi A, Nagata K
    • Journal Title

      Front. Microbiol

      Volume: 4 Pages: 398-398

    • NAID

      120007130538

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The N-terminal region of influenza virus polymerase PB1 adjacent to the PA binding site is involved in replication but not transcription of the viral genome.2013

    • Author(s)
      Binh NT, Wakai C, Kawaguchi A, Nagata K.
    • Journal Title

      Frontiers in Microbiology

      Volume: 4 Pages: 398-398

    • NAID

      120007130538

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Critical Role of an Antiviral Stress Granule Containing RIG-I and PKR in Viral Detection and Innate Immunity2012

    • Author(s)
      Onomoto K, Jogi M, Yoo J-S, Narita R, Morimoto S, Takemura A, Sambhara S, Kawaguchi A, Osari S, Nagata K, Matsumiya T, Namiki H, Yoneyama M, Fujita T
    • Journal Title

      PLoS One

      Volume: 7 Issue: 8 Pages: 1-15

    • DOI

      10.1371/journal.pone.0043031

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] YB-1 functions as a porter to lead influenza virus ribonucleoprotein complexes to microtubules2012

    • Author(s)
      Kawaguchi A, Matsumoto K, Nagata K
    • Journal Title

      J. Virol

      Volume: 86(20) Pages: 11086-11095

    • NAID

      120007130530

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] YB-1, Y-box binding protein-1, is a porter to lead influenza virus ribonucleoprotein complexes to microtubules.2012

    • Author(s)
      Kawaguchi, A., Matsumoto, K., and Nagata, K.
    • Journal Title

      Journal of Virology

      Volume: 86 Issue: 20 Pages: 11086-11095

    • DOI

      10.1128/jvi.00453-12

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] ウイルスポリメラーゼによって規定されるトリインフルエンザウイルスの哺乳類細胞への適応機構2013

    • Author(s)
      原田芳美、川口敦史、永田恭介
    • Organizer
      平成25年度日本生化学会関東支部例会
    • Place of Presentation
      甲府
    • Year and Date
      2013-06-15
    • Related Report
      2013 Final Research Report
  • [Presentation] ポリコーム複合体因子であるEZH2はインフルエンザウイルスタンパク質M1およびインフルエンザウイルスゲノムの核外輸送を制御する2013

    • Author(s)
      浅賀正充、川口敦史、永田恭介
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] インフルエンザウイルス感染に応答した宿主因子YB-1による中心体の機能制御2013

    • Author(s)
      川口敦史、永田恭介
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] インフルエンザポリメラーゼ遺伝子間の適合性2013

    • Author(s)
      原田芳美、川口敦史、永田恭介
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] インフルエンザウイルスゲノムの細胞内輸送機構におけるNS1の新規機能2013

    • Author(s)
      長利卓、川口敦史、永田恭介
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Harald Wodrich, Nagata K, Michael Kann. In cellulo analysis of parvovirus - mediated changes of nuclear envelope integrity by real time microscopy2013

    • Author(s)
      Kenza Snoussi, Kawaguchi A, Kato K
    • Organizer
      Leading Graduate Schools International Conference (Tsukuba Global Science Week)
    • Place of Presentation
      Tsukuba
    • Related Report
      2013 Final Research Report
  • [Presentation] YB-1 is a porter to lead influenza virus ribonucleoprotein complexes to microtubules for the virus production2013

    • Author(s)
      Kawaguchi A
    • Organizer
      The 2^<nd> Meeting on RNA and Biofunctions-Asia Study"RNA Biofunctions and Viruses"
    • Place of Presentation
      Fukuoka
    • Related Report
      2013 Final Research Report
  • [Presentation] In cellulo analysis of parvovirus-mediated changes of nuclear envelope integrity by real time microscopy.2013

    • Author(s)
      Kenza Snoussi, Kawaguchi A, Kato K, Harald Wodrich, Nagata K, Michael Kann.
    • Organizer
      Leading Graduate Schools International Conference
    • Place of Presentation
      つくば
    • Related Report
      2013 Annual Research Report
  • [Presentation] YB-1 is a porter to lead influenza virus ribonucleoprotein complexes to microtubules.2013

    • Author(s)
      Kawaguchi A, Nagata K.
    • Organizer
      15th International Negative Strand Virus Meeting 2013.
    • Place of Presentation
      グラナダ(スペイン)
    • Related Report
      2013 Annual Research Report
  • [Presentation] YB-1 is a porter to lead influenza virus ribonucleoprotein complexes to microtubules for the virus production.2013

    • Author(s)
      Kawaguchi A.
    • Organizer
      The 2nd Meeting on RNA and Bionfunctions-Asia Study "RNA Biofunctions and Viruses"
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] YB-1 is a porter to lead influenza virus ribonucleoprotein complexes to microtubules2012

    • Author(s)
      Kawaguchi A, Nagata K
    • Organizer
      The 3rd Leading Graduate Schools International Conference (Tsukuba Global Science Week)
    • Place of Presentation
      Tsukuba
    • Related Report
      2013 Final Research Report
  • [Presentation] A novel function of NS1 viral protein in intracellular of the influenza virus genome2012

    • Author(s)
      Osari S, Kawaguchi A, Nagata K
    • Organizer
      The 7th Tsukuba Medical Science Research Meeting (Tsukuba Global Science Week)
    • Place of Presentation
      Tsukuba
    • Related Report
      2013 Final Research Report
  • [Presentation] 新規宿主因子YB-1によるインフルエンザウイルスゲノムの細胞内輸送制御2012

    • Author(s)
      川口敦史、松本健、永田恭介
    • Organizer
      第60回日本ウイルス学会学術集会
    • Place of Presentation
      大阪
    • Related Report
      2013 Final Research Report 2012 Annual Research Report
  • [Presentation] 新規宿主因子YB-1によるインフルエンザウイルスRNP複合体の微小管への輸送機構解析2012

    • Author(s)
      川口敦史、松本健、永田恭介
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
  • [Presentation] EZH2はインフルエンザゲノムの核外輸送を制御する2012

    • Author(s)
      浅賀正充、川口敦史、永田恭介
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
  • [Presentation] YB-1 is a porter to lead influenza virus ribonucleoprotein complexes to microtubules.2012

    • Author(s)
      Kawaguchi A.
    • Organizer
      The 3rd Leading Graduate Schools International Conference
    • Place of Presentation
      つくば
    • Related Report
      2012 Annual Research Report
  • [Presentation] A novel function of NS1 viral protein in intracellular of the influenza virus genome.2012

    • Author(s)
      Osari S.
    • Organizer
      The 7th Tsukuba Medical Science Research Meeting
    • Place of Presentation
      つくば
    • Related Report
      2012 Annual Research Report
  • [Presentation] 新規宿主因子YB-1によるインフルエンザウイルスRNP複合体の微小管への輸送機構解析2012

    • Author(s)
      川口敦史
    • Organizer
      第35回日本分子生物学会年回
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] EZH2はインフルエンザウイルスゲノムの核外輸送を制御する2012

    • Author(s)
      浅賀正充
    • Organizer
      第35回日本分子生物学会年回
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] ウイルスポリメラーゼによって規定されるトリインフルエンザウイルスの哺乳類細胞への適応機構

    • Author(s)
      原田芳美、 川口敦史、 永田恭介
    • Organizer
      平成25年度日本生化学会関東支部例会
    • Place of Presentation
      甲府
    • Related Report
      2013 Annual Research Report
  • [Remarks]

    • URL

      http://www.md.tsukuba.ac.jp/basic-med/infectionbiology/virology/

    • Related Report
      2013 Final Research Report
  • [Remarks] 川口研究室ホームページ

    • URL

      http://www.md.tsukuba.ac.jp/basic-med/kawaguchilab/

    • Related Report
      2012 Annual Research Report

URL: 

Published: 2012-11-27   Modified: 2023-03-16  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi