| Project/Area Number |
24890256
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemical pharmacy
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
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| Project Period (FY) |
2012-08-31 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | がん治療薬 / イリジウム錯体 / 燐光 / ペプチド / pHプローブ / イメージング / 細胞死 / 金属含有がん治療薬 / アポトーシス / TRAIL / デスリガンド / デスレセプター |
|---|
| Research Abstract |
TNF-related apoptosis-inducing ligand (TRAIL) having a C3-symmetric homotrimeric structure induces the apoptosis in various tumor cells and tissues through the interaction with death receptor (DR) on cell surface. In this study, we have addressed the design and synthesis of new C3-symmetric tris-cyclometalated Ir complexes having dual activity both agonist activity for apoptosis induction and luminescent imaging of cancer cells.Staining experiments of Jurkat cell with Ir complex having the DR5 binding cyclic peptide suggested that green emission was localized on the cell membrane. Moreover, flow cytometer analysis suggested that the correlation between DR5 expressions of cancer cells (Jurkat cells, Molt-4 cells, K562 cells) and emission intensities derived from Ir complex. Optimizations of Ir complexes for improvement of their TRAIL-like activity are undergoing.
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